高等学校化学学报

高等学校化学学报 ›› 1998, Vol. 19 ›› Issue (9): 1363.

• 论文 • 上一篇    下一篇

Cu(phen)22+和Cu(bpy)22+与6-巯基嘌呤、腺嘌呤相互作用的研究

张荣丽, 朱俊杰, 赵广超, 陈洪渊   

  1. 南京大学化学系, 配位化学国家重点实验室, 南京, 210093
  • 收稿日期:1997-08-26 出版日期:1998-09-24 发布日期:1998-09-24
  • 通讯作者: 陈洪渊
  • 作者简介:张荣丽,女,36岁,高级访问学者,讲师.
  • 基金资助:

    国家自然科学基金(批准号:29775010)和江苏省自然科学基金资助课题.

Studies on Interaction of Copper Complexes of Cu(phen) 22+ and Cu(bpy) 22+ with 6-Mercaptopurine and Adenine

ZHANG Rong-Li, ZHU Jun-Jie, ZHAO Guang-Chao, CHEN Hong-Yuan   

  1. Department of Chemistry, Nanjing University, State Key Laboratory of Coordination Chemistry, Nanjing, 210093
  • Received:1997-08-26 Online:1998-09-24 Published:1998-09-24

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摘要： 在pH为7.0的磷酸盐缓冲溶液中,用荧光光谱、紫外光谱、电化学及紫外光谱电化学等方法研究了铜配合物与6-巯基嘌呤、腺嘌呤的相互作用.结果表明,Cu(phen)22+和Cu(bpy)22+与6-巯基嘌呤、腺嘌呤发生了相互作用,但作用程度不同.根据荧光光谱实验数据计算出Cu(phen)22+和Cu(bpy)22+与6-巯基嘌呤、腺嘌呤的配位比均为1:1;它们与6-巯基嘌呤作用的配位常数分别为2.23×104L/mol和6.11×104L/mol;与腺嘌呤作用的配位常数分别为1.95×104L/mol和5.12×104L/mol.电化学实验也获得了相近的结果.这为解释Cu(pllea)22+和Cu(bpy)22+与DNA的作用机理及作用部位提供了有益的信息.

关键词: 6-巯基嘌呤, 腺嘌呤, 铜配合物, 荧光光谱, 紫外-可见光谱, 光谱电化学

Abstract: In this paper, the interaction of complexes Cu(phen) 22+ and Cu(bpy) 22+ with 6-mercaptopurine (6-MP) and adenine were studied by fluorescence, UV-Vis spectroelectrochemical, and spectroelectrochemical methods. Fluorescence and electrochemical results indicated that the complex ratios of copper complexes with 6-MPand adenine are 1:1, and the complex constants of Cu(phen) 22+ and Cu(bpy) 22+ with 6-MPare 2.23×104 L/mol and 6.11 ×104 L/mol, with adenine being 1.91×104 L/mol and 5.45×104 L/mol respectively. Spectroelectrochemical behavior of the copper complexes changed obviously in the presence of 6-MP.These results indicated that there are strong interactions of copper complexes with 6-MP.

Key words: 6-Mercaptopurine, Adenine, Copper complex, Fluorescence spectrometry, UV-Vis spectrum, Spectroelectrochemistry

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