高等学校化学学报

高等学校化学学报 ›› 1997, Vol. 18 ›› Issue (4): 615.

• 论文 • 上一篇    下一篇

聚邻苯二胺膜电极中辣根过氧化物酶的电子传递

陈滨晖, 朱侃, 吴辉煌, 张瀛洲   

  1. 厦门大学化学系固体表面物理化学国家重点实验室, 厦门, 361005
  • 收稿日期:1996-04-18 出版日期:1997-05-24 发布日期:1997-05-24
  • 通讯作者: 吴辉煌.
  • 作者简介:陈滨晖, 男, 26岁, 硕士研究生.
  • 基金资助:

    国家自然科学基金;国家教育委员会博士点基金

Electron Transfer of Horseradish Peroxidase Entrapped in Poly-o-phenylenediamine Membrane Electrode

CHEN Bin-Hui, ZHU Kan, WU Hui-Huang, ZHANG Ying-Zhou   

  1. Department of Chemistry, State Key Laboratory of Physical Chemistryfor the Solid Surfaces, Xiamen University, Xiamen, 361005
  • Received:1996-04-18 Online:1997-05-24 Published:1997-05-24

PDF (PC)

被引次数

10

摘要： 利用酶的电化学固定化方法制备合辣根过氧化物酶的聚邻苯二胺膜电极,研究其伏安行为及对H2O2还原的生物电催化作用.结果表明,在所述生物电催化反应中酶与聚合物基质发生了直接电子传递.但对新制的酶电极而言,电聚合时生成并包理在酶膜中的寡聚体可作为电子传递体加速氧化态酶的再生.根据酶电极电流响应实验曲线的拟合,发现氧化态酶的再生速度随电极电位的变化表观上符合Tafel关系式.提出了酶反应动力学参数的测定方法.

关键词: 辣根过氧化物酶, 聚邻苯二胺, 生物电催化, 电流式酶电极

Abstract: The poly-o-phenylenediamine membrane electrode containing horseradish peroxi-dase was prepared by means of the electrochemical immobilization of enzyme, and thevoltammetric behavior of the resulting enzyme electrode and its bioelectrocatalysis to hydro-gen peroxide reduction was studied.It was found that the direct electron tansfer between theenzyme and the polymer matrix takes place in the biocatalytic processes.For the newly pre-pared enzyme electrode, however, the oligomers, which are formed during the polymeriza-tion of o-phenylenediamine and then incorporated in the enzyme membrane, can play a role ofelectron transfer mediators to accelerate the regeneration of the oxidized enzyme.From thefitting of experimental current response curves, it can be found that the change in the regen-eration rates of the oxidized enzyme with the electrode potentials obeys apparantly the Tafelrelationship.The procedure in the measurement of kinetic parameters for enzyme reactionswas suggested.

Key words: Horseradish peroxidase, Poly-o-phenylenediamine, Bioelectrocatalysis, Amperometric enzyme electrodes

TrendMD: