高等学校化学学报 ›› 1996, Vol. 17 ›› Issue (6): 953.

• 论文 • 上一篇    下一篇

微胶囊固定化过氧化氢酶

王玉洁, 刘持标, 白光月, 褚莹, 王凤平, 马占芳, 张河哲   

  1. 东北师范大学化学系, 长春 130024
  • 收稿日期:1995-06-22 出版日期:1996-06-24 发布日期:1996-06-24
  • 通讯作者: 王玉洁,男,36岁,硕士,副教授.
  • 作者简介:王玉洁,男,36岁,硕士,副教授.
  • 基金资助:

    国家自然科学基金重点基金

Immobilized Catalase with Microcapsules

WANG Yu-Jie, LIU Chin-Biao, BAI Guang-Yue, CHU Ying, WANG Feng-Ping, MA Zhan-Fang, ZHANG He-Zhe   

  1. Department of Chemistry, Northeast Normal University, Changchun 130024
  • Received:1995-06-22 Online:1996-06-24 Published:1996-06-24

摘要: 以乙基纤维素为膜材,用液中干燥法将过氧化氢酶微胶囊化。研究了温度和pH值对酶活性的影响及过氧化氢和尿素对固定化酶的抑制作用。由于乙基纤维素膜的保护,明胶对pH值的缓冲作用及明胶与尿素的反应,明显地扩大了酶对温度和pH值的适应性,降低了酶活性抑制剂的影响。

关键词: 微胶囊, 过氧化氢酶, 固定化酶

Abstract: Catalase was microencapsulated with ethyl cellulose as a film material by a method of drying in liquid.It was investigated that the immobilized catalase was affected by temperature and pHand inhibited by hydrogen peroxide.The available temperature and pHranges of the microencapsulated catalase owing to the protection of ethyl cellulose film and the buffer action of gelatine and phosphoric acid separately,and the inhibition, because of the presence of gelatine,are enlarged clearly.

Key words: Microcapsule, Catalase, Immobilized enzyme

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