关键词: 填料, 高效阳离子交换色谱, 性能, 蛋白质

Abstract: The packings of cation exchange chromatography with phosphono group and matrix of silica for separation of proteins have been synthsised, and the relationship between their structure and characteristics has been studied.This kind of packings of cation exchange chromatography with medium intensity has a high recovery of the bioactivity of proteins,better separation function and wider applied scope than those of the weak exchange chromatography in the previous papers.The protein load capacity of these packings is about 20 mg/mLfor a sample of Lysozyme by the dynamic load capacity determination method.The use scope of pH is 3.5 to 8. The bioactivity recovery of Lysozyme is 96.7%.It is noteworthy that the hydrophobicity of ligand has an effect on the retention of proteins in IEC. With the increase of the ligand hydrophobicity, the retention of proteins increases. On the other hand, the change of the salt concentration in the mobile phase can change the hydrophobic action between proteins and packings, therefore change the retention of proteins. When two proteins have a great hydrophobic difference and a near retention in the IEC, maybe the change of the salt concentration can get a good separation result.

Key words: Packing, High performance cation exchange chromatography, Characteristics, Protein