高等学校化学学报

高等学校化学学报 ›› 1994, Vol. 15 ›› Issue (7): 1042.

• 论文 • 上一篇    下一篇

辣根过氧化物酶的电化学固定化及其对H2O2的生物电催化还原作用

杨防祖, 许书楷, 吴辉煌, 周绍民   

  1. 厦门大学物理化学研究所, 化学系, 厦门, 361005
  • 收稿日期:1993-07-09 修回日期:1994-04-09 出版日期:1994-07-24 发布日期:1994-07-24
  • 通讯作者: 杨防祖,男,32岁,助理研究员.
  • 作者简介:杨防祖,男,32岁,助理研究员.
  • 基金资助:

    国家自然科学基金

Electrochemical Immobilization of Horse-Radis Peroxidase and Its Bioelectrocatalysis in H2O2 Redudion

YANG Fang-Zu, XU Shu-Kai, WU Hui-Huang, Zhou Shao-Min   

  1. Institute of Physical Chemistry, Department of Chemistry, Xiamen University, Xiamen, 361005
  • Received:1993-07-09 Revised:1994-04-09 Online:1994-07-24 Published:1994-07-24

PDF (PC)

摘要： 利用电化学固定化方法制备了聚吡咯/辣根过氧化物酶(PP/HRP)膜电极,并研究了其电化学行为。在除氧的磷酸盐缓冲液介质中,PP/HRP电极加速H2O2的还原,归因于酶加成物的直接电子传递。探索HRP与电子传递体K4Fe(CN)6在聚吡咯(PP)膜中的同时固定化条件及其膜电极的电化学行为,实验证实,K4Fe(CN)6在酶膜中的存在使得H2O2的还原电位强烈正移,在-0.05V的工作电位下能对H2O2进行检测,相应的电极过程可用间接氧化还原催化机理解释。

关键词: 辣根过氧化物酶, 酶电极, 电化学固定化, 生物电催化还原

Abstract: With electropolymerization of pyrrole, horse-radish peroxidase(HRP) was immobilized in the polypyrrole matrix to construct PP/HRPenzyme electrode, and its electrochemical behaviour was studied.In the deoxygenated phosphate buffer, the PP/HRPelectrode showed an accelerated H2O2 reduction, which was attributed to the direct electron transfer of the adduct of HRPwith H2O2.The simultaneous immobilization of HRPand K4Fe(CN)6 into the polypyrrole(PP) polymer and the electrochemical behaviour of as-formed enzyme electrodes were investigated.It was confirmed that, the existence of K4Fe(CN)6 in the polymer enabled the reduction potential of H2O2 to strongly shift to positive so that H2O2 could be detected at-0.05 V.The related process was explained in terms of indirect redox catalysis.

Key words: Horse-radish peroxidase, Enzyme electrode, Electrochemical immobilization, Bioelectrocatalytic reduction

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