高等学校化学学报 ›› 1994, Vol. 15 ›› Issue (12): 1770.

• 研究快报 • 上一篇    下一篇

酶联放大镧系螯合物发光法─辣根过氧化物酶(HRP)-铽-对羟基苯甲酸体系的研究及用于HRP和结核抗体的分析

谢剑炜, 鄢远, 彭学军, 许金钩, 陈国珍   

  1. 厦门大学化学系, 厦门, 361005
  • 收稿日期:1994-05-09 修回日期:1994-06-20 出版日期:1994-12-24 发布日期:1994-12-24
  • 通讯作者: 陈国珍.
  • 作者简介:谢剑炜,男,30岁,讲师,博士研究生.

Enzyme-Amplified Lanthanide Luminescence Spectrometry─Studies on Horseradish Peroxidase-Terbium-p-Hydroxybenzoic Acid System and Its Application to the Determination of Horseradish Peroxidase and Tuberculosis Antibody

XIE Jian-Wei, YAN Yuan, PENG Xue-Jun, XU Jin-Gou, CHEN Guo-Zhen   

  1. Department of Chemistry and Institute of Analytical Chemistry, Xiamen, 361005
  • Received:1994-05-09 Revised:1994-06-20 Online:1994-12-24 Published:1994-12-24

关键词: 酶联放大镧系螯合物发光法, 辣根过氧化物酶, 结核抗体

Abstract: Enzyme-amplified lanthanide luminescence (EALL)is a new method which has been recently developed for enzymatically amplified signal detection in ultrasensitive bioanalytical assays where an enzyme is used as label or analysed by itself. In the paper, Horseradish peroxidase (HRP)-Terbium-p-hydroxybenzoic acid system has been studied.The EALLmethod has also been demonstrated for the detection of HRPand tuberculosis antibody.

Key words: Enzyme-amplified lanthanide luminescence spectrometry, Horseradish peroxidase, Tuberculosis antibody

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