高等学校化学学报

高等学校化学学报 ›› 1992, Vol. 13 ›› Issue (2): 227.

• 论文 • 上一篇    下一篇

酶的光谱电化学研究(Ⅰ)——辣根过氧化物酶的电化学性质

吴宝璋, 吴辉煌, 周绍民   

  1. 厦门大学化学系, 厦门, 361005
  • 收稿日期:1991-01-09 出版日期:1992-02-24 发布日期:1992-02-24
  • 通讯作者: 吴辉煌
  • 基金资助:

    国家自然科学基金

Spectroelectrochemical Studies of Enzymes (Ⅰ)——Electrochemical Properties of Horseradish Peroxidase

WU Bao-zhang, WU Hui-huang, ZHOU Shao-min   

  1. Department of Chemistry, Xiamen University, Xiamen, 361005
  • Received:1991-01-09 Online:1992-02-24 Published:1992-02-24

PDF (PC)

摘要： 利用薄层光谱电化学技术研究了辣根过氧化物酶(HRP)及其化合物的氧化还原过程。指出HRP可在固体电极上进行直接电子传递,该电极反应不是酶中二硫键的还原,而是血红素辅基中心金属离子的氧化态转变。测定了HRP(Fe3+)/Fe2+))电对的标准氧化还原电位和电化学动力学参数,讨论了HRP氧化性中间物的电化学性质。

关键词: 辣根过氧化物酶, 生物氧化还原, 透光薄层电极, 光谱电化学

Abstract: The redox processes of horseradish peroxidase (HRP) and its compounds were investigated by the thin-layer spectroelectrochemical techniqueIt is shown that the direct electron transfer of the enzyme HRPon the solid electrodes can be carried out and the electrode reaction is the oxidation-state transformation of the central metal ion in prosthetic group heme rather than the reduction of disulfide group in HRPmoleculeThe standard redox potential of HRP(Fe3+/ Fe2+) couple and electrochemical kinetic parameters were measured, and the electrochemical properties of HRP compound Iwere also discussed.

Key words: Horseradish peroxidase, Biological redox process, Optically transparent thin-layer electrode (OTTLE), Spectroelectrochemistry

TrendMD: