高等学校化学学报 ›› 1991, Vol. 12 ›› Issue (9): 1272.

• 论文 • 上一篇    下一篇

聚乙烯醇高含水胶固定化脲酶的研究

潘继伦, 陈长治, 俞耀庭   

  1. 南开大学分子生物学研究所, 天津, 300071
  • 收稿日期:1990-06-22 出版日期:1991-09-24 发布日期:1991-09-24
  • 通讯作者: 潘继伦

Studies on the Behavior of Urease Immobilized by Cross-Linked Polyvinyl Alcohol Gel

Pan Ji-lun, Chen Chang-zhi, Yu Yao-ting   

  1. Institute for Molecular Biology, Nankai Univeristy, Tianjin, 300071
  • Received:1990-06-22 Online:1991-09-24 Published:1991-09-24

摘要: 冷冻-部分脱水法制成的聚乙烯醇高含水胶固定化脲酶活力收率及对脲素的分解能力明显高于聚丙烯酰胺囱定化脲酶,稳定性相近;用低浓度的戊二醛后处理,提高了固定化酶的稳定性。

关键词: 聚乙烯醇高含水胶, 脲酶, 酶活力收率, 稳定性, 固定化

Abstract: The present paper reports the immobilization of urease on "cross-linked" PVAgel containing a high amount of water. Experimented results show that the immobilized urease on PVAgel has a high activity yield(79%). The immobilized urease activity is 63 mg/g (half hour at 37℃). The repeated determination with substrate showed no detectable loss in enzymatic activity. The determination of the activity within 26days at 37℃ showed a 26% loss of enzymatic activity.

Key words: PVA gel, Urease, Yield of enzymatic activity, Stability, Immobilization

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