高等学校化学学报 ›› 1991, Vol. 12 ›› Issue (5): 695.

• 论文 • 上一篇    下一篇

溶解性可调节的酶载体制备和固定化酶的研究

李增吉1, 沈家骢1, 马林2, 程玉华2   

  1. 1. 吉林大学化学系, 130023;
    2. 吉林大学酶工程国家开放实验室
  • 收稿日期:1989-12-11 出版日期:1991-05-24 发布日期:1991-05-24
  • 通讯作者: 李增吉

Preparation of Enzyme Carriers with Regulated Solubility and Studies on Immobilized Enzyme

Li Zeng-ji1, Shen Jia-cong1, Ma Lin2, Cheng Yu-hua2   

  1. 1. Department of Ctiemistry, Jilin University, Changchun, 130023;
    2. National Open Laboratory of Enzyme Engineering, Jtiin University, Changchun
  • Received:1989-12-11 Online:1991-05-24 Published:1991-05-24

摘要: 本文利用自由基沉淀聚合反应,合成了甲基丙烯酸-丙烯酰胺-顺丁烯二酸酐三元共聚物,测定了这些共聚物形成水不溶性的大分子氢键复合物的临界pH值.利用共聚物上的酸酐基团,直接进行了木瓜蛋白酶的固定化,得到了具有液相酶与固相酶两者优点的新型修饰酶。

关键词: 大分子复合物, 可逆沉淀-溶解性, 固定化酶

Abstract: The methacrylic acid-acrylamide-maleic anhydride copolymers were prepared by radical precipitate copolymerization in dioxane. The copolymers exhibit the property of reversible precipitation-solubility changes due to their macromolecular complexes formed by hydrogen bonds when the pHvalues of the solution were changed. The immobilization of papain was carried out by using the copolymer as a carrier without other coupling agents. The immobilized papain shows the insoluble form below pH 3. 8 and soluble form above pH 3. 9. The immobilized papain with the advantages of soluble and in-soluble enzyme was obtained.

Key words: Macromolecular complex, Regulated solubility, Immobilized enzyme

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