高等学校化学学报

高等学校化学学报 ›› 1989, Vol. 10 ›› Issue (3): 289.

• 研究论文 • 上一篇    下一篇

几种修饰剂共价修饰L-门冬酰胺酶降低其抗原性的对比研究

曹淑桂, 田洁, 王妮, 程玉华   

  1. 吉林大学酶工程实验室
  • 收稿日期:1987-07-21 出版日期:1989-03-24 发布日期:1989-03-24
  • 通讯作者: 曹淑桂

Influences of Modifiers and Methods of Modification on Activity and Antigenicity of 1-Asparaginase

Cao Shugui, Tian Jie, Wang Ni, Cheng Yuhua   

  1. Enzyme Engineering Laboratory, Jilin University, Changchun
  • Received:1987-07-21 Online:1989-03-24 Published:1989-03-24

PDF (PC)

被引次数

7

摘要： 选用单甲氧基聚乙二醇、右旋糖苷、磺化右旋糖苷,肝素及小分子醋酸酐对酶分子进行了共价修饰,研究了不同修饰剂对酶分子的酶活力及其抗原性的影响。结果表明,大分子,特别是单甲氧基聚乙二醇的降低抗原性效果很好,但活力损失较大;修饰剂分子量越大,酶表面氨基修饰程度越高,解除抗原性效果越好。

关键词: L-门冬酰胺酶, PEG, 醋酸酐, 化学修饰剂

Abstract: Small and macromolecular modifiers and different methods were used for modification of E. coli L-asparaginase to reduce the antigenicity.The results showed that the macromolecular modifiers are better than that of small molecular modifier acetic anhydride for reduction of antigenicity.Acomparison of the modified L-asparaginase by PEG, dextran,dextran sulfate and heparin showed that there is a relationship between the rate of modified free amino groups and the reduction of antigenicity.

Key words: L-asparaginase, PEG, Acetic anhydride, Chemical modification

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