高等学校化学学报

高等学校化学学报 ›› 1989, Vol. 10 ›› Issue (10): 999.

• 研究论文 • 上一篇    下一篇

PVA固定化脲酶电极的制作及电极特性的研究

陈佩琴, 谢伟文, 林思聪   

  1. 南京大学化学系
  • 收稿日期:1988-01-08 出版日期:1989-10-24 发布日期:1989-10-24
  • 通讯作者: 陈佩琴

Making and Characteristics of a PVA Immobilized Urease Electrode

Chen Peiqin, Xie Weiwen, Lin Sicong   

  1. Department of Chemistry, Nanjing University, Nanjing
  • Received:1988-01-08 Online:1989-10-24 Published:1989-10-24

PDF (PC)

被引次数

1

摘要： 利用PVA低温下的物理交联固定酶,制成酶电极并在不同尿素浓度、pH值、温度等条件下进一步研究这种酶电极的响应特性.

关键词: 脲酶, 固定化脲酶膜, 酶电极, 尿素, 氨气敏电极

Abstract: Aurease electrode immobilized with polyvinyl alcohol hydrogel (PVA) was made and its response characteristics were studied under different concentrations of urea (Curea) and at different temperatures and in a buffer solutiont with different pHvalues.It is revealed that for this kind of urease electrode the highest response speed is at 30℃ and pH 9.3, and the maximum steady state potential is at 33℃ ; meanwhile, there exist linear relationships of both v～lg Curea and E～lg Curea in the urea concentration ranges of 3.4× 10-4～2.0× 10-2 mol·L-1 and 2.0×10-4～3.7×10-2 mol·L-1, respectively.

Key words: Urease, Immobilized urease, Enzyme electrode, Urea, Ammonia gas sensing electrode

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