高等学校化学学报 ›› 2020, Vol. 41 ›› Issue (3): 411-416.doi: 10.7503/cjcu20190681

• 庆祝《高等学校化学学报》复刊40周年专栏 • 上一篇    下一篇

聚集诱导发光分子/适配体/外切酶Ⅰ体系对可卡因的检测

杜宪超1,郝红霞2,*,秦安军1,*,唐本忠1,3   

  1. 1. 华南理工大学发光材料与器件国家重点实验室, 广东省分子聚集发光重点实验室, 聚集诱导发光研究中心, 广州 510640
    2. 中国政法大学证据科学教育部重点实验室, 2011计划司法文明协同创新中心, 北京 100088
    3. 香港科技大学化学系, 国家人体组织重建工程技术研究中心香港分中心, 香港
  • 收稿日期:2019-12-17 出版日期:2020-03-10 发布日期:2020-02-10
  • 通讯作者: 郝红霞,秦安军
  • 作者简介:郝红霞, 女, 博士, 教授, 主要从事毒化理化分析研究. E-mail: hongxiah@cupl.edu.cn|秦安军, 男, 博士, 教授, 主要从事基于三键单体的高分子合成化学及有机聚合物光电功能材料方面的研究. E-mail:msqinaj@scut.edu.cn
  • 基金资助:
    国家自然科学基金(Nos.21788102);国家自然科学基金(Nos.21525417);国家自然科学基金(Nos.21490571);广东省自然科学基金(Nos.2019B030301003);广东省自然科学基金(Nos.2016A030312002);香港创新及科技基金资助(No.ITC-CNERC14SC01)

Detection of Cocaine Based on the System of AIEgen, Aptamer and Exonuclease Ⅰ

DU Xianchao1,HAO Hongxia2,*,QIN Anjun1,*,TANG Benzhong1,3   

  1. 1. State Key Laboratory of Luminescent Materials and Devices, Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates, Center for Aggregation-Induced Emission, South China University of Technology, Guangzhou 510640, China
    2. Collaborative Innovation Center of Judicial Civilization, Key Laboratory of Evidence Science of Ministry of Education, China University of Political Science and Law, Beijing 100088, China
    3. Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, The Hong Kong University of Science & Technology, Hong Kong, China
  • Received:2019-12-17 Online:2020-03-10 Published:2020-02-10
  • Contact: Hongxia HAO,Anjun QIN
  • Supported by:
    † Supported by the National Natural Science Foundation of China(Nos.21788102);† Supported by the National Natural Science Foundation of China(Nos.21525417);† Supported by the National Natural Science Foundation of China(Nos.21490571);the Natural Science Fund of Guangdong Province, China(Nos.2019B030301003);the Natural Science Fund of Guangdong Province, China(Nos.2016A030312002);the Innovation and Technology Commission of Hong Kong, China(No.ITC-CNERC14SC01)

关键词: 聚集诱导发光, 可卡因, 适配体, 外切酶Ⅰ

Abstract:

A new system for detection of cocaine was designed based on an AIEgen of TPE-TTA, anticocaine aptamer and exonuclease Ⅰ(Exo Ⅰ) . The Exo Ⅰ can specifically cleave the phosphodiester bonds of nucleotides in nucleic acids either from the ends or interior of single stranded DNA(ssDNA). Meanwhile, the cocaine can combine with anticocaine aptamer and switch its configuration to Y-shaped double stranded DNAs(dsDNA) that prevent the aptamer degradation in the presence of Exo Ⅰ. When cationic 4,4',4″,4″'-{[ethene-1,1,2,2-tetrayltetrakis(benzene-4,1-diyl)]tetrakis(oxy)}-tetrakis(N,N,N-triethylbutan-1-aminium) bromide(TPE-TTA) was added to the Tris-HCl solution, it can combine with aptamer and the fluore-scence was turned on. The experiment conditions, including the amount of Exo Ⅰ, incubation time of the Exo Ⅰ with anticocaine aptamer, incubation temperature and time of anticocaine aptamer with cocaine were systematically investigated and the optimal conditions were obtained. With different concentrations of cocaine, the experimental results showed that the fluorescence intensity was progressively enhanced. The limit of detection(LOD) of 0.66 μmol/L was deduced. Moreover, the developed method also was used to determine cocaine in real urine samples, and the LOD can reach as low as 1.0 μmol/L. This work provides a new method for cocaine detection, which is promising for practical application.

Key words: Aggregation-induced emission, Cocaine, Aptamer, Exonuclease Ⅰ

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