高等学校化学学报 ›› 2019, Vol. 40 ›› Issue (8): 1593-1599.doi: 10.7503/cjcu20190243

• 分析化学 • 上一篇    下一篇

基于碳点的荧光纳米开关灵敏检测Cu(Ⅱ)离子和焦磷酸盐

周思慧, 李琼, 张婷, 庞代文, 唐宏武   

  1. 生物医学分析化学教育部重点实验室, 武汉大学化学与分子科学学院, 武汉 430072
  • 收稿日期:2019-04-26 修回日期:2019-07-23 出版日期:2019-08-10 发布日期:2019-08-02
  • 通讯作者: 唐宏武,男,博士,教授,博士生导师,主要从事生物光子学检测技术和纳米生物传感等方面的研究.E-mail:hwtang@whu.edu.cn E-mail:hwtang@whu.edu.cn
  • 基金资助:
    国家自然科学基金(批准号:81572086,81772256,21827808)资助.

Luminescent Nanoswitch Based on Carbon Dots for Sensitive Detection of Cu(Ⅱ) Ions and Pyrophosphates

ZHOU Sihui, LI Qiong, ZHANG Ting, PANG Daiwen, TANG Hongwu   

  1. Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China
  • Received:2019-04-26 Revised:2019-07-23 Online:2019-08-10 Published:2019-08-02
  • Supported by:
    Supported by the National Natural Science Foundation of China(Nos.81572086, 81772256, 21827808).

摘要: 利用羟基与氨基在高温条件下反应,不断聚合生成碳点(CDs),该碳点可发射不依赖激发波长的明亮的红色荧光.将CDs作为目标敏感荧光团时,发现Cu2+可特异性猝灭碳点的荧光,而焦磷酸盐(PPi)可在一定程度上恢复上述体系的荧光.其中,Cu2+对CDs的荧光猝灭是由于Cu2+与CDs发生络合反应,从而发生静态猝灭过程;而加入PPi之后,由于它与CDs的结合能力更强,因此Cu2+离开CDs的表面,体系的荧光得以恢复.在此基础上构建了一种高选择性、高灵敏度的off-on荧光纳米开关传感体系,分别用于Cu2+和PPi的定量检测,检出限分别达2.14 μmol/L和1.85 μmol/L.该传感体系可应用于实际样品检测,拓宽了荧光CDs的传感应用,为其在生物体内目标分子的检测提供了可能性.

关键词: 碳点, 荧光传感, Cu2+检测, 焦磷酸盐

Abstract: Carbon dots(CDs) with brightly red fluorescence were prepared via a facile one-step synthesis way by hydroxyl and amino groups to react at high temperature. The as-obtained CDs can overcome the excitation-dependence luminescence behavior for normal routes and the quantum yield is up to 41%. Based on the fluorescent materials, a luminescent nanoswitch through fluorescence on and off in response to specific external stimuli was designed. Fluorescence quenching of CDs by Cu2+ is ascribed to the complexation reaction. When pyrophosphate(PPi) is introduced into the system, Cu2+ leaves CDs' surface because PPi binds to CDs much stronger than Cu2+, resulting in the fluorescence recovery. Following the proposed way, a signal off-on nanoswich sensing system for the detection of Cu2+ and PPi with high selectivity and sensitivity was constructed, and the detection limits for Cu2+ and PPi are 2.14 μmol/L and 1.85 μmol/L, respectively. This method has been applied in real samples, and this strategy broadens the sensing application of fluorescent CDs, providing the possibility to determine these targets in living systems.

Key words: Carbon dots, Fluorescence sensor, Cu2+ detection, Pyrophosphate

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