高等学校化学学报 ›› 2018, Vol. 39 ›› Issue (3): 591-597.doi: 10.7503/cjcu20170450

• 高分子化学 • 上一篇    下一篇

大豆分离蛋白/琼脂糖复合水凝胶

刘杰1, 周浩2,*(), 黄郁芳3, 陈新1,*()   

  1. 1. 聚合物分子工程国家重点实验室, 复旦大学高分子科学系,先进材料实验室, 上海 200433
    2.复旦大学附属眼耳鼻喉科医院, 上海 200031
    3. 复旦大学材料科学系, 上海 200433
  • 收稿日期:2017-07-11 出版日期:2018-03-10 发布日期:2018-01-13
  • 通讯作者: 周浩,陈新 E-mail:zheent@139.com;chenx@fudan.edu.cn
  • 作者简介:联系人简介: 陈 新, 男, 博士, 教授, 博士生导师, 主要从事生物大分子材料研究. E-mail: chenx@fudan.edu.cn; 周 浩, 男, 副教授, 副主任医师, 主要从事眼科临床及生物医用材料研究. E-mail: zheent@139.com
  • 基金资助:
    国家自然科学基金(批准号: 21274028, 21574023, 21574024)资助

Soy Protein Isolate/Agar Composite Hydrogel

LIU Jie1, ZHOU Hao2,*(), HUANG Yufang3, CHEN Xin1,*()   

  1. 1. State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Laboratory of Advanced Materials, Fudan University, Shanghai 200433, China
    2. Eye and ENT Hospital, Fudan University, Shanghai 200031, China
    3. Department of Materials Science, Fudan University, Shanghai 200433, China
  • Received:2017-07-11 Online:2018-03-10 Published:2018-01-13
  • Contact: ZHOU Hao,CHEN Xin E-mail:zheent@139.com;chenx@fudan.edu.cn
  • Supported by:
    † Supported by the National Natural Science Foundation of China(Nos.21274028, 21574023, 21574024)

摘要:

采用简单的物理共混方法, 制备一种大豆分离蛋白/琼脂糖降温型复合水凝胶. 即在高温下聚乙二醇改性的大豆分离蛋白/琼脂糖混合物呈溶液状态, 降温至生理温度37 ℃或更低温度则形成水凝胶. 表征了该复合凝胶的溶胀度、 力学性能、 细胞毒性和细胞迁移率. 结果表明, 改性大豆分离蛋白/琼脂糖复合水凝胶具有较高的溶胀度和力学性能, 良好的生物相容性和细胞迁移率, 在伤口敷料领域显示出潜在的应用.

关键词: 改性大豆分离蛋白, 琼脂糖, 复合水凝胶, 伤口敷料

Abstract:

Agar, a plant polysaccharide, was selected to form composite hydrogel with pure and polyethylene glycol(PEG)-modified soy protein isolates(SPI) by a simple blending method. It was found that SPI/agar mixture was in solution state at high temperature but quickly formed a hydrogel after cooling down to the physiological temperature(37 ℃) or lower. Therefore, such a natural polymer-based thermo-sensitive hydrogel can be designed into different shapes. SEM images indicated that PEG-modified SPI/agar hydrogel had more compact network structure than the pure SPI/agar hydrogel. The swelling ratio, mechanical properties, cytotoxicity, and cell migration ability of both pure and PEG-modified SPI/agar composite hydrogel were extensively investigated. Compared with the pure agar hydrogel and pure SPI/agar composite hydrogel, PEG-modified SPI/agar hydrogel showed the largest swelling ratio(higher than 1600%). In addition, the PEG-modified SPI/agar composite hydrogels showed good mechanical properties, which was comparable to that of pure agar hydrogel. In vitro cell experiments demonstrated that PEG-modified SPI/agar hydrogel had both good biocompatibility and high cell migration ability. Therefore, all excellent properties shown above made the PEG-modified SPI/agar hydrogel a good candidate for wound dressing.

Key words: Polyethylene glycol-modified soy protein isolate, Agar, Composite hydrogel, Wound dressing

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