高等学校化学学报 ›› 2016, Vol. 37 ›› Issue (11): 1972.doi: 10.7503/cjcu20160499

• 有机化学 • 上一篇    下一篇

分离自木霉菌Trichoderma sp.的新型化合物Trichoderol A及绝对构型的确定

许兰兰1, 赵齐齐1, 俞和1,2, 王景晨1, 王慧君1, 杨芹1, 朱华结1(), 李艳3   

  1. 1. 药物化学与分子诊断教育部重点实验室, 河北省药物质量分析控制重点实验室,河北大学药学院, 保定 071002
    2. 机械工业分析仪器产品质量监督检测中心, 北京 100095
    3. 中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室, 昆明 650204
  • 收稿日期:2016-07-13 出版日期:2016-11-10 发布日期:2016-09-26
  • 作者简介:联系人简介: 朱华结, 男, 博士, 教授, 博士导师, 主要从事手性药物化学研究. E-mail: jackzhu2002@sina.com
  • 基金资助:
    2012河北省巨人计划、 保定市科技支撑项目(批准号: 15ZN020)、 河北省教育厅资助科研项目(批准号: QN2016177)和国家自然科学基金(批准号: 41606174)资助

Absolute Configuration Determination of One New Compound Trichoderol A from Trichoderma sp. Fungus

XU Lanlan1, ZHAO Qiqi1, YU He1,2, WANG Jingchen1, WANG Huijun1, YANG Qin1, ZHU Huajie1,*(), LI Yan3   

  1. 1. Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of Pharmaceutical Sciences,Hebei University, Baoding 071002, China
    2. Machinery Industry Analytical Industry Quality Supervision and Testing Centre, Beijing 100095, China
    3. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany,Kunming 650204, China
  • Received:2016-07-13 Online:2016-11-10 Published:2016-09-26
  • Contact: ZHU Huajie E-mail:jackzhu2002@sina.com
  • Supported by:
    † Supported by the 2012 Giant Project of Hebei Province, China, the Science and Technology Support Project of Baoding, China(No.15ZN020), the Scientific Research Foundation of Hebei Educational Committee, China(No;QN2016177) and the National Natural Science Foundation of China(No.41606174)

摘要:

采用经典柱色谱方法从土壤真菌木霉菌Trichoderma sp.中分离得到1个新的化合物, 为长链不饱和醇类. 运用核磁共振及质谱等手段确定了化合物的平面结构, 再结合电子圆二色谱(ECD)、 旋光(OR)及量子化学方法计算确定其绝对构型. 结果表明, 该新化合物为Trichoderol A(1). 活性测试结果显示, 该化合物对3株致病菌具有一定程度的抗菌活性(MIC=25 μmol/L).

关键词: Trichoderma sp., 电子圆二色光谱, 旋光, 矩阵模型, 抗菌活性

Abstract:

One new compound, trichoderol A(1), was isolated from cultures of the fungus Trichoderma sp. The structure of compound 1 was established by means of NMR and mass spectrometry. The absolute configuration(AC) was determined by means of comparing experimental electronic circular dichroism(ECD) and optical rotation(OR) of compound 1 with the calculated ECD and OR data of compound 1 using quantum methods, also using matrix model. Compound 1 was evaluated for antibacterial activities against three species of pathogenic bacteria, and the results showed that compound 1 had weak antibacterial activities(MIC=25 μmol/L).

Key words: Trichoderma sp., Electronic circular dichroism, Optical rotation, Matrix, Antibacterial activity

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