高等学校化学学报 ›› 2014, Vol. 35 ›› Issue (5): 1057.doi: 10.7503/cjcu20130859

• 物理化学 • 上一篇    下一篇

不同阴离子组成的离子液体对产紫青霉生长、代谢及催化特性的影响

李扬1,2, 曹红1,2(), 欧阳巧凤2, 李春3, 郑兰兰2, 张馨2   

  1. 1. 嘉兴学院生物与化学工程学院, 嘉兴 314001
    2. 石河子大学化学化工学院, 新疆兵团化工绿色重点实验室-省部共建国家重点实验室培育基地, 石河子 832003
    3. 北京理工大学生命学院, 北京 100081
  • 收稿日期:2013-09-03 出版日期:2014-05-10 发布日期:2014-04-12
  • 作者简介:联系人简介: 曹 红, 女, 博士, 副教授, 主要从事生物催化与酶工程的研究. E-mail:chyf_ch@126.com
  • 基金资助:
    国家自然科学基金项目(批准号: 21146012, 21266029)、 教育部科学技术研究重点项目(批准号: 209146)、 新疆生产建设兵团博士资金(批准号: 2009JC13)和石河子大学高层次人才科研启动资金专项(批准号: RCZX200805)资助

Effects of Ionic Liquids with Different Anion Composition on Cell Growth, Metabolism and Catalysis Characteristics of Penicillium purpurogenum Li-3

LI Yang1,2, CAO Hong1,2,*(), OUYANG Qiaofeng2, LI Chun3, ZHENG Lanlan2, ZHANG Xin2   

  1. 1. College of Biological and Chemical Engineering, Jiaxing University, Jiaxing 314001, China
    2. School of Chemistry and Chemical Engineering, Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Shihezi University, Shihezi 832003, China
    3. School of Life Science, Beijing Institute of Technology, Beijing 100081, China
  • Received:2013-09-03 Online:2014-05-10 Published:2014-04-12
  • Contact: CAO Hong E-mail:chyf_ch@126.com
  • Supported by:
    † Supported by the National Natural Science Foundation of China(Nos.21146012, 21266029), the Science and Technology Foundation of Ministry of Education, China(No.209146), the Doctoral Foundation of Ministry of Formation(No.2009JC13) and the High Level Talent Research of Ministry of Shihezi University(No;RCZX200805)

摘要:

研究了6种不同阴离子分别与1-丁基-3-甲基咪唑阳离子[Bmim]+组成的6种离子液体对产紫青霉(Penicillium purpurogenum Li-3)细胞生长、 代谢、 细胞膜透性和催化活性的影响. 结果表明, [Bmim]Ac和[Bmim]Tf2N对菌体细胞的生长和代谢具有明显的抑制作用; 6种离子液体均改善了细胞膜透性, 其中以[Bmim]Ac最明显; 全细胞催化反应实验表明, 在分别含25%的疏水性离子液体[Bmim]PF6和[Bmim]Tf2N的2种介质体系中, 甘草酸(GL)生物转化合成单葡萄糖醛酸基甘草次酸(GAMG)的转化率分别为82.73%和89.84%, GAMG的产率分别为68.65%和76.23%, 与不含离子液体的纯缓冲溶液体系相比(GL转化率和GAMG产率分别为81.82%和33.31%), GL的转化率均略有提高, 而GAMG的产率则提高了2倍多, 表明2种疏水性离子液体对全细胞催化反应的定向性具有明显促进作用; 亲水性离子液体[Bmim]Ac, [Bmim]Cl和[Bmim]NO3对细胞的催化活性具有明显的抑制作用, 而[Bmim]BF4的抑制作用则较弱.

关键词: 离子液体, 阴离子, 产紫青霉, 全细胞, 催化特性

Abstract:

The wild-type Penicillium purpurogenum Li-3 strain is capable of expressing highly β-glucuronidase, a intracellular enzyme with excellent ability to biotransform glycyrrhizin(GL) into glycyrrhetinic acid monoglucuronide(GAMG) possessing best biological function. To improve the production of GAMG, the effects of ionic liquids with different anion composition on Penicillium purpurogenum Li-3 cells properties of growth, metabolism, permeability of cell membrane and catalysis were studied in this paper. The results showed that [Bmim]Ac and [Bmim]Tf2N had obvious inhibitory effects on cell growth and metabolism. Six kinds of ionic liquids all improved the permeability of cell membrane, and [Bmim]Ac was the most remarkable. In the two catalytic medium respectively containing 25% hydrophobic [Bmim]PF6 and [Bmim]Tf2N, conversion rates of substrate GL were 82.73% and 89.84%, and the yields of GAMG were 68.65% and 76.23%, respectively. Thus, the substrate GL conversion increased slightly, but the yield of GAMG were about two times higher than that in the pure buffer solution without ionic liquids(conversion rate of GL 81.82%, yield of GAMG 33.31%). It was obvious that hydrophobic [Bmim]PF6 and [Bmim]Tf2N were capable of promoting the selectivity of the whole cell catalysis. While hydrophilic [Bmim]Ac, [Bmim]Cl and [Bmim]NO3 obviously had inhibition in different degrees on the cell’s catalytic activity. These results indicated that the anions in ionic liquids played a crucial role as biological catalytic medium.

Key words: Ionic liquid, Anion, Penicillium purpurogenum Li-3, Whole cell, Catalytic property

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