NaYF4∶Yb3+, Er3+上转换发光纳米晶在三元混合溶剂体系中的配体交换修饰及发光性质

doi:10.7503/cjcu20130779

高等学校化学学报 ›› 2014, Vol. 35 ›› Issue (2): 224.doi: 10.7503/cjcu20130779

• 研究论文: 无机化学 • 上一篇下一篇

NaYF₄∶Yb³⁺, Er³⁺上转换发光纳米晶在三元混合溶剂体系中的配体交换修饰及发光性质

张庆彬¹(), 孔祥贵², 王新³, 程成¹

1. 浙江工业大学激光与光电子技术研究所, 杭州 310023
2. 中国科学院长春光学精密机械与物理研究所激发态重点实验室, 长春 130033
3. 中国科学院苏州纳米技术与纳米仿生研究所, 苏州 215123

收稿日期:2013-08-12 出版日期:2014-02-10 发布日期:2013-12-05
作者简介:联系人简介: 张庆彬, 男, 博士, 讲师, 主要从事纳米光电材料制备与光学检测研究. E-mail:qbzhang@zjut.edu.cn
基金资助:
国家自然科学基金(批准号: 21305126)和浙江工业大学校自然科学基金(批准号: 2012XZ003)资助

NaYF₄∶Yb³⁺,Er³⁺ Upconverting Nanoparticles Surface Ligand Exchange in Ternary Mixture Solvent and Optical Properties^†

ZHANG Qingbin^1,^*(), KONG Xianggui², WANG Xin³, CHENG Cheng¹

1. Institute of Laser and Optoelectronic Technology, Zhejiang University of Technology, Hangzhou 310023, China
2. Key Laboratory of Excited State Process, Changchun Institute of Optics, Fine Mechanics and Physics,Chinese Academy of Sciences, Changchun 130033, China
3. Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, Suzhou 215123, China

Received:2013-08-12 Online:2014-02-10 Published:2013-12-05
Contact: ZHANG Qingbin E-mail:qbzhang@zjut.edu.cn
Supported by:
† Supported by the National Natural Science Foundation of China(No.21305126) and the Natural Science Foundation of Zhejiang University of Technology, China(No.2013X2003)

摘要/Abstract

摘要：

选用2-胺乙基膦酸双官能团小分子作为配体交换剂, 采用氯仿/乙醇/水三元混合溶剂体系下的配体交换修饰方法, 解决了疏水纳米晶与亲水配体的有效接触反应问题, 实现了上转换纳米晶的水溶性以及表面胺基功能化修饰. 通过傅里叶变转换红外光谱和热重分析证实了表面配体分子的替换. 透射电子显微镜和激光粒度分析结果显示, 所得水溶性纳米晶具有粒径均一, 水力直径小和分散稳定的特点. 样品的发射光谱结果表明, 配体交换过程对纳米晶的发光无明显影响, 保持了良好的发光性能. 通过荧光共聚焦成像技术实现了胺基修饰上转换发光纳米晶对HeLa细胞的光学成像, 证实所得纳米晶适合于潜在的生物学应用.

关键词: 上转换发光纳米晶, 2-胺乙基膦酸, 配体交换

Abstract:

Hydrophobic up-converting rare-earth nanoparticles(UCNPs) were transferred from organic solvent to aqueous solution via a simple surface-ligand exchange procedure. 2-Aminoethyl dihydrogen phosphate(AEP) molecules were used to replace the original hydrophobic ligands in chloroform, ethanol and water ternary mixture solvent at ambient temperature. The ligand exchange process was confirmed by Fourier transform infrared spectra(FTIR) and thermogravimetric analysis(TG). Investigations by transmission electron microscopy(TEM), dynamic light scattering(DLS) and photoluminescence(PL) spectroscopy show no obvious variations in the morphologies, structure and luminescentproperties of the UCNPs during the ligand exchange process. The fluorescence confocal cell imaging of AEP modified hydrophilic UCNPs confirms that the hydrophilic UCNPs are suitable for potential biological labeling.

Key words: Upconversion nanoparticle, 2-aminoethyl dihydrogen phosphate(AEP), Ligand exchange

中图分类号:

O616

TrendMD:

张庆彬, 孔祥贵, 王新, 程成. NaYF₄∶Yb³⁺, Er³⁺上转换发光纳米晶在三元混合溶剂体系中的配体交换修饰及发光性质. 高等学校化学学报, 2014, 35(2): 224.

ZHANG Qingbin, KONG Xianggui, WANG Xin, CHENG Cheng. NaYF₄∶Yb³⁺,Er³⁺ Upconverting Nanoparticles Surface Ligand Exchange in Ternary Mixture Solvent and Optical Properties^†. Chem. J. Chinese Universities, 2014, 35(2): 224.

图/表 8

Fig.1 Schematic illustration of the general principle of the ligand exchange approach using 2-aminoethyl dihydrogenphosphate substitutional ligands

Fig.2 TEM images of NaYF4∶Yb3+,Er3+ UCNPs before(A) and after(B) ligand exchange

Fig.3 Hydrodynamic diameter distribution of the AEP coated NaYF4∶Yb3+, Er3+ UCNPs in water

Fig.4 FTIR spectra of NaYF4∶Yb3+,Er3+ UCNPs before(a) and after(b) ligand exchange using AEP

Fig.5 TGA curves of NaYF4∶Yb3+, Er3+ UCNPs before(a) and after(b) ligand exchange

Fig.6 Luminescence spectra of NaYF4∶Yb3+,Er3+ UCNPs in chloroform(a) and AEP capped sample in water(b)

Fig.7 Fluorescence image of NaYF4∶Yb3+,Er3+ NCs dissolved in water without excitation(A) and continuous-wave excitation at 980 nm(B)

Fig.8 Confocal fluorescence images of the HeLa cell (A) Bright-field images; (B) the dark-field images.

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NaYF₄∶Yb³⁺, Er³⁺上转换发光纳米晶在三元混合溶剂体系中的配体交换修饰及发光性质

NaYF₄∶Yb³⁺,Er³⁺ Upconverting Nanoparticles Surface Ligand Exchange in Ternary Mixture Solvent and Optical Properties^†

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