高等学校化学学报 ›› 2019, Vol. 40 ›› Issue (7): 1405-1410.doi: 10.7503/cjcu20190129

• 有机化学 • 上一篇    下一篇

姜黄素对食管癌KYSE410细胞的 生长抑制与氧化调节

周新媛, 洪佳馨, 何少兵, 张连茹()   

  1. 细胞应激生物学国家重点实验室, 厦门大学生命科学学院, 厦门361102
  • 收稿日期:2019-02-28 出版日期:2019-06-28 发布日期:2019-07-09
  • 作者简介:

    张连茹, 女, 博士, 教授, 主要从事微生物药物研究. E-mail: ru898@xmu.edu.cn

  • 基金资助:
    国家自然科学基金(批准号: 81373296)、 细胞应激生物学国家重点实验室(厦门大学)开放课题基金(批准号: SKLCSB2017KF003)和厦门大学大学生创新创业训练计划项目资助.

Effects of Curcumin on Growth and Oxide Regulation of Esophageal Cancer KYSE410 Cells

ZHOU Xinyuan, HONG Jiaxin, HE Shaobing, ZHANG Lianru*()   

  1. State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen 361102, China
  • Received:2019-02-28 Online:2019-06-28 Published:2019-07-09
  • Contact: ZHANG Lianru E-mail:ru898@xmu.edu.cn
  • Supported by:
    † Supported by the National Natural Science Foundation of China(No.81373296), the Open Research Fund of State Key Laboratory of Cellular Stress Biology, Xiamen University, China(No.SKLCSB2017KF003) and the Xiamen University Undergraduate Innovation and Entrepreneurship Training Programs, China.

摘要:

通过四氮唑蓝盐化合物(MTS)和重要氧化还原酶及其代谢物的试剂盒检测等方法, 考察了姜黄素对食管癌KYSE410细胞生长以及对细胞氧化还原状态和代谢的影响. 结果表明, 姜黄素对KYSE410细胞具有较强的抑制作用, 其IC50=17.9 μmol/L. 进一步研究发现, 姜黄素可引起细胞培养上层清液中超氧化物歧化酶(SOD)和丙二醛(MDA)水平发生变化. 当姜黄素浓度为40 μmol/L时, MDA水平比对照组提高了125.1%, 而SOD水平则降低了43.2%; 同时, 乳酸水平降低了44.4%, 乳酸脱氢酶的活性下降了58.2%, 丙酮酸激酶的活性升高了216.7%. 姜黄素可能通过干扰氧化还原途径, 致使发生脂质过氧化反应, 并抑制肿瘤细胞糖酵解作用, 进而抑制食管癌KYSE410细胞增殖.

关键词: 姜黄素, 食管癌, KYSE410细胞

Abstract:

This work took esophageal cancer KYSE410 cells as the research object. [3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium(MTS) method and some other techniques were used to study the effect of curcumin on growth and metabolism of KYSE410 cells. The results show that curcumin strongly inhibited growth of KYSE410 cells and half maximal inhibitory concentration(IC50) was 17.9 μmol/L. Besides, the level of superoxide dismutase(SOD) decreases to 43.2% and the malondialdehyde(MDA) increases to 125.1%. Also, the level of lactate decreases to 44.4% and the activity of lactate dehydrogenase decreases to 58.2% and the activity of pyruvate kinase increases to 216.7%. It is presumed that curcumin has strong suppressed effect on this kind of cell. Curcumin can interfere oxidative stress and inhibit glycolysis by decreasing activity of lactate dehydrogenase and increasing activity of pyruvate kinase.

Key words: Curcumin, Esophageal cancer, KYSE410 cell

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