The Effect of the Buffer pH in Capillary Zone Electrophoresis

Chem. J. Chinese Universities ›› 1993, Vol. 14 ›› Issue (9): 1229.

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The Effect of the Buffer pH in Capillary Zone Electrophoresis

LIN Bing-Cheng¹, SHI Wei¹, HU Liu-Jiang², YANG Guo-Liang², HUANG Jian-Fu³, ZHANG Nai-Qian³

1. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116012;
2. Dept.of Chem., Hangzhou Univ., Hangzhou;
3. Dalian Inst.of Light Industry, Dalian

Received:1992-09-30 Revised:1993-05-31 Online:1993-09-24 Published:1993-09-24

Abstract

Abstract: The effect of buffer pHon the migration time of both peptides and proteins was studied in the range from pH 2.5 to 111 the dependence of the elute order and resolution of the components on the pHis very sensitive and should be optimized.Special attention was also paied to it's effect on the absorption of the wall on the proteins at the basic condition.It is one of the ways to inhibit the absorption, but the pHvalues should be chosen very carefully.

Key words: Capillary zone electrophoresis, Effect of pH, Peptide, Protein, Absorption

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LIN Bing-Cheng, SHI Wei, HU Liu-Jiang, YANG Guo-Liang, HUANG Jian-Fu, ZHANG Nai-Qian. The Effect of the Buffer pH in Capillary Zone Electrophoresis[J]. Chem. J. Chinese Universities, 1993, 14(9): 1229.

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The Effect of the Buffer pH in Capillary Zone Electrophoresis

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