Abstract: A composition from the cultured body of [Hericium caput-medusae(Bull.: Fr.) Pers.] was analyzed quantitatively and qualitatively by gas chromatography-mass spectrometry(GC-MS) and liquid chromatography-tandem mass spectrometry(LC-MS/MS). Di-n-octyl o-phthalate(DOP) and di-n-butyl o-phthalate(DBP)were identified by manually mass spectrum analyzing and mass spectrum bank matching. Since plastics are not inert, the plasticizers might migrate into the cultured mushroom and cause contamination. A simple LC-MS/MS method was established for the determination of nine plasticizers in cultured edible mushroom due to potential contamination. The method shows a good recovery(71.6%—82.2%) for various levels of spiked samples(0.01—0.10 mg/L).The relative standard deviations were in the range of 2.11%—8.53% for all plasticizers studied. The limits of detection were in the range of 0.002—0.30 μg/L.

Key words: Hericium caput-medusae(Bull Fr.) Pers., Plasticizer, LC-MS/MS