Chem. J. Chinese Universities

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Bioeffects of Silica-coated Nanoparticles on COS-7 Cells

LIU Fang, HE Xiao-Xiao, WANG Ke-Min*, GE Jia, TAN Wei-Hong   

  1. State Key Laboratory of Chemo/Biosensing and Chemometrics, Engineering Center for Biomedicine, Institute of Life Science & Biological Technology, Key Laboratory for Bio-Nanotechnology and Molecule Engineering of Hunan Province, Changsha 410082, China
  • Received:2007-01-25 Revised:1900-01-01 Online:2007-10-10 Published:2007-10-10
  • Contact: WANG Ke-Min

Abstract: In this paper, the cell livability, cell cycle and cell growth curve were analyzed to evaluate the bioeffects of silica-coated nanoparticles on COS-7 cell line. The results indicate that the cytotoxicity of FSiNPs on COS-7 cells was concentration dependent. No measurable cytotoxic effects of FSiNPs were observed on the cell livability, cell cycle and cell growth if the nanoparticles concentration below 0.2 μg/μL. But the cytoto-xic of FSiNPs on COS-7 cells would increase with the increasing of nanoparticles concentration. Meanwhile, the cellular uptake of FSiNPs at low concentration by COS-7 cells was also examined by employing the fluorescence dye RuBpy doped as a signal for tracking the silica coated nanoparticles. It was found that cells can uptake FSiNPs at random by cell membranous' endocytosis when they co-cultured together, and then they excluded parts of FSiNPs to cell culture medium, also parts of FSiNPs can be distributed to the next generation cells. Moreover, the content of FSiNPs in cells are less and less following the manifold of new cytoplasm and cells reproduction, while cells still remained a good shape and growth state after the uptake of nanoparticels. These results provide safely standards for the research, analysis and application of FSiNPs in cell biology. Also, it was a foundation of the research of bionano-device based on the novel nanoparticles.

Key words: Silica coated nanoparticles(SiNPs), COS-7 cell line, Bioeffects

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