Abstract: Techniques from microcalorimetry and transmission electron microscopy(TEM) were employed to investigate the metabolic effects and the ultrastructural changes of human body carcinoma of ovary cell line(COC₁) at different temperatures with and without synergetic effects of anti tumor medicine(HCFU). The mechanism of hyperthemia and chemotherapy in the treatment of the tumor cells was explored at both macroscopic and microscopic levels. The results showed that the metabolic activity of the tumor cells was reduced at high temperature and the cell morphology changed malignantly. High temperature might induce apoptosis of the tumor cells; and the combined effects of hyperthemia and chemotherapy could speed up the apoptosis and reduce the metabolic power of the tumor cells. Asustained longer time action could lead to ultimate death of the tumor cells.

Key words: Microcalorimetry, TEM, High temperature, Tumor cell, Apoptosis