Abstract:

Electrochemical techniques were employed to study the electrochemical behavior in a wider pH range, the antioxidative ability and the interaction with DNA of resveratrol. The experimental results show that wave P₁ detected in 2.0<pH<7.1 solutions was an irreversible reduction wave of resveratrol involving 2e and 2H⁺; and wave P₃ detected in 7.3<pH<10.9 solutions was caused by the same reason with P₁, wave P₂ located at less negative potential was produced by the reversible reduction of ionized resveratrol involving 2e and 2H⁺; as expected, the reduction waves P₄ and P₅ of bivalent and trivalent anion of further ionized resveratrol also present in the solution with pH>11. Under the optimum conditions, a linear relationship could be established between the peak current of wave P₃ and the concentration of resveratrol in the range from 8.0×10^-8 to 2.0×10^-6 mol/L, and the detection limit was 4×10^-8 mol/L. The determination result of content of resveratrol in Chinese traditional herbal medicine Polygonum cuspidatum by the proposed differential pulse polarography method was consistent with the result by the comparison HPLC method. The antioxidative a
bility of resveratrol and the interaction mode of resveratrol with DNA were prov
ed by electrochemical method.

Key words: Electrochemical behavior, Resveratrol, Reduction wave, Polygonum cuspidatum, DNA