A New Biochemical Method for Protein Separation Based on Gold Nanotubule Membrane

Abstract

Abstract: Gold nanotubule membranes with about 55 nm of diameter were prepared by chemical deposition of gold on polycarbonate templates membrane. After being modified by cysteine and guanide thiocyanate, the nanomembranes were studied with BSA and IgG as the model molecules. The results show that guanide thiocyanate facilitated transporting of BSA by 30 to 50 times because of hydrophilic and denaturalization effects whereas IgG almost retained its′ transporting speed. Then the separation processes were carried out and separation degrees of 31.6 and 23.1 were obtained for cysteine and guanide thiocyanate modified gold nanotubules, respectively at pH 4.5. The results indicate that gold nanotubule membranes has a good separating capability for protein.

Key words: Gold nanotubule membrane, Protein, Transporting, Separation

CLC Number:

O657

TrendMD:

HUANG Shan-Sheng, YIN Yue-Fen, WANG Ke-Min, HE Xiao-Xiao, ZHONG Tong-Sheng . A New Biochemical Method for Protein Separation Based on Gold Nanotubule Membrane[J]. Chem. J. Chinese Universities, 2004, 25(12): 2238.

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A New Biochemical Method for Protein Separation Based on Gold Nanotubule Membrane

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