Interaction Between Lysozyme and Three Flavones by Fluorescence Spectroscopy

Chem. J. Chinese Universities

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Interaction Between Lysozyme and Three Flavones by Fluorescence Spectroscopy

YANG Ran¹, CHEN Xiao-Lan², LI Ping¹, QU Ling-Bo²

1. Key Laboratory of Modern Chinese Medicines, Ministry of Education, China Pharmaceutical University, Nanjing 210038, China;
2. Key Laboratory of Chemical Biology, Department of Chemistry, Zhengzhou University, Zhengzhou 450052, China

Received:2005-08-03 Revised:1900-01-01 Online:2006-09-10 Published:2006-09-10
Contact: QU Ling-Bo

Abstract

Abstract: The interaction between lysozyme and three flavones, including Apigenin, Luteocin and Quercetin, were studied by fluorescence spectroscopy. The influence of C3—OH and C3'—OH on the interaction of flavones-Lys were compared. The results showed that C3'—OH could enhance the binding affinity greatly; while C3—OH weakened the affinity largely. According to the structure parameters of assay flavones, the effect of hydrogen group on the flavones-protein were discussed, and the main reason resulting in the weaker binding affinity of Quercetin-Lys were explored. The results obtained from the work could supply beneficial reference for the further study of Flavone-Protein interactions.

Key words: Fluorescence spectroscopy, Lysozyme, Apigenin, Luteocin, Quercetin, Energy transfer, Structure parameter

CLC Number:

O625
O641

TrendMD:

YANG Ran¹, CHEN Xiao-Lan², LI Ping¹, QU Ling-Bo². Interaction Between Lysozyme and Three Flavones by Fluorescence Spectroscopy[J]. Chem. J. Chinese Universities, doi: .

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Interaction Between Lysozyme and Three Flavones by Fluorescence Spectroscopy

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