Chem. J. Chinese Universities ›› 2017, Vol. 38 ›› Issue (12): 2238.doi: 10.7503/cjcu20170359

• Physical Chemistry • Previous Articles     Next Articles

Effect of Bovine Serum Albumin on the Structure of DSPE Monolayer

XU Guoqing, HAO Changchun*(), HE Jianzhen, ZHANG Lei, SUN Runguang*()   

  1. College of Physics and Information Technology, Shaanxi Normal University, Xi’an 710062, China
  • Received:2017-06-07 Online:2017-12-10 Published:2017-10-10
  • Contact: HAO Changchun,SUN Runguang E-mail:haochangchun@snnu.edu.cn;biophymed@snnu.edu.cn
  • Supported by:
    Supported by the National Natural Science Foundation of China(No.21402114), the Natural Science Basic Research Plan of Shaanxi Province, China(No.2016JM2010), the Fundamental Research Funds for the Central Universities of China(Nos.2017CSY004, GK201603026) and the National University Science and Technology Innovation Project of China(No.CX17189)

Abstract:

The effect of bovine serum albumin(BSA) on the 1,2-distearoyl-sn-glycero-3-phosphoethanolamine(DSPE) monolayer at air-water interface was investigated by Langmuir-blodgett(LB) technique and atomic force microscopy(AFM) observation. The surface pressure-mean molecular area(π-A) isotherms, adsorption curves and compression-expansion cycle curves of DSPE monolayers were obtained by changing the pH values and the concentration of BSA. The results indicated that the presence of BSA in the subphase had a great influence on the compressibility, stability and phase transition behavior of DSPE monolayers. The adsorption kinetic indicated that BSA was adsorbed onto DSPE monolayers and a threshold value existed, which was affected by the pH value. Analysis of experimental data showed that most hydrophobic residues of BSA were exposed to water at pH=3, so the interaction between BSA and DSPE was strongest. However, the interaction was the weakest at pH=7 because less hydrophobic residues were exposed to water. The change of surface morphology structure observed by AFM was consistent with the results obtained from curves. This study provides an important experimental basis and theoretical basis for understanding the interaction mechanism between serum proteins and phospholipid molecules.

Key words: Bovine serum albumin(BSA), 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine(DSPE), Surface pressure, Interaction

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