Chem. J. Chinese Universities ›› 2017, Vol. 38 ›› Issue (12): 2193.doi: 10.7503/cjcu20170232

• Organic Chemistry • Previous Articles     Next Articles

Fucoidan Degradation and Preparation of Fuco-oligosaccharides from Saccharina japonica

GENG Lihua1,2,3, JIN Weihua1, WANG Jing1,2, ZHANG Quanbin1,3,*()   

  1. 1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences,Qingdao 266071, China
    2. University of Chinese Academy of Sciences, Beijing 100049, China
    3. Lab for Marine Biology and Biotechnology, Qingdao National Lab for Marine Science and Technology,Qingdao 266071, China
  • Received:2017-04-13 Online:2017-12-10 Published:2017-11-20
  • Contact: ZHANG Quanbin
  • Supported by:
    Supported by the National Natural Science Foundation of China(No.41506165), the Focus on Research and Development Plan in Shandong Province(Marine Medical Food), China(No.2016YYSP002), the Shandong Provincial Natural Science Foundation of China(No.ZR2014DQ024) and the Science and Technology Program of Applied Basic Research Projects of Qingdao Municipal, China(No.15-9-1-74-jch)


The sulfated fuco-oligosaccharides were prepared via acid hydrolysis degradation of crude fucoidan extracted from Saccharina japonica. Through the optimization of degradation and precipitation conditions, the hydrolysis and sedimentation law of fucoidan were studied. Physicochemical analysis showed that the supernatant was heterozygous sulfated fuco-oligosaccharide(fucose>55%). Four fractions of fucose based low polyme-rization degree of oligosaccharides were purified by Bio-Gel P4 low-pressure permeation chromatography, and the fucose content of the fractions was up to 90% according to monosaccharide composition analysis and electrospray ionization mass spectrometry(ESI-MS) analysis. Thus, the fractions were series of low-polymerized sulfated fuco-oligosaccharide/fucose, and the major fractions could be expressed with the formula of Fuc1~3S1~3, Fuc1~2S1~2, Fuc1~2S, and FucGlcAS.

Key words: Fucoidan, Fuco-oligosaccharide, Acid hydrolysis

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