Dynamic Observation of Cellular Localization of Fluorescein Isothiocyanate Labeled Recombinant Ganoderma Lucidum Immunoregulatory Protein(rLz-8) in NB4 APL Cell

• 研究论文 • Previous Articles Next Articles

Dynamic Observation of Cellular Localization of Fluorescein Isothiocyanate Labeled Recombinant Ganoderma Lucidum Immunoregulatory Protein(rLz-8) in NB4 APL Cell

LIANG Chong-Yang¹, XU Wei-Qing², CAO Yan-Xin², LIU Li-Xia³, ZHANG Shu-Qin¹, LIU Zhi-Yi¹, LI Hong-Rui¹, LI Bai-Zhi¹, SUN Fei^1*

1. Institute of Frontier Medical Science, Jilin University, Changchun 130021, China;
2. State Key Lab for Supramolecular Structure and Materials, Jilin University, Changchun 130012, China;
3. School of Life Sciences, Northeast Normal University, Changchun 130024, China

Received:2008-07-21 Revised:1900-01-01 Online:2009-03-10 Published:2009-03-10
Contact: SUN Fei

Abstract

Abstract: NB4 APL cell was induced apoptosis by Recombinant Ganoderma lucidum immunoregulatory protein(rLz-8) for the first time in this study. The dynamic cellular localization of FITC-rLz-8 was observed by laser scanning confocal microscope LSCM: identifying the receptors of cell membrane, entering into cell, gathering on nucleus finally. Annexin V-FITC kit was used for measuring apoptosis of NB4 by rLz-8. Results show that the effects of the dose group(0.5 μg/mL) is close to the one of positive control(As₂O₃). All data show that apoptosis inducing activity of rLz-8 in HL60 cells and changes of cell morphology and Ca²⁺ level in cytosol are probably interrelate with rLz-8's subcellular localization.

Key words: Fluorescein isothiocyanate(FITC), Subcellular localization, Nucleus, Apoptosis, NB4 cell

CLC Number:

TrendMD:

LIANG Chong-Yang¹, XU Wei-Qing², CAO Yan-Xin², LIU Li-Xia³, ZHANG Shu-Qin¹, LIU Zhi-Yi¹, LI Hong-Rui¹, LI Bai-Zhi¹, SUN Fei^1*
. Dynamic Observation of Cellular Localization of Fluorescein Isothiocyanate Labeled Recombinant Ganoderma Lucidum Immunoregulatory Protein(rLz-8) in NB4 APL Cell[J]. Chem. J. Chinese Universities, doi: .

[1]	YUAN Jiayi, YE Baotong, WU Jing, LI Ying, CHEN Jinghua, CHEN Jingxiao. Study on Sulfated Glycosylated Fe₃O₄@SiO₂ Nanoparticles Inducing Tumor Cells Apoptosis^† [J]. Chem. J. Chinese Universities, 2018, 39(11): 2458.
[2]	ZHANG Jinliang, MA Xin, XU Mengxi, ZONG Cheng, REN Bin. Study on Apoptosis Process of CaSki via Fast Line-scanning Raman Imaging^† [J]. Chem. J. Chinese Universities, 2016, 37(7): 1257.
[3]	ZHANG Tao, TANG Yongjia, XU Liang, LIU Keliang. Synthesis of a New Cyanoacrylate Monomer and Its Application in Fluorescence Imaging in vivo^† [J]. Chem. J. Chinese Universities, 2016, 37(6): 1168.
[4]	DING Guobin, LI Binchun, GUO Yi, XU Li. Spectral Properties and Antitumor Activity of 10-Hydroxycamptothecin and Its Application in Cell Labeling^† [J]. Chem. J. Chinese Universities, 2014, 35(11): 2324.
[5]	SHI Sheng-Gang, FENG Ji-Kang, TIAN Wei-Quan, LIU Zi-Zhong, LI Wei-Qi, CUI Yan-Hong. Theoretical Studies on Structures and Aromaticity of NiTi Clusters [J]. Chem. J. Chinese Universities, 2013, 34(2): 455.
[6]	ZHU Zhi-Jie, LIU Qiong, CHEN Ping, XU Xu, NI Jia-Zuan, YANG Si-Lin, SONG Yun. Seleno-polymannuronate Synthesis and Resistance to Oxidation and Apoptosis in Alzheimer’s Disease Cells [J]. Chem. J. Chinese Universities, 2013, 34(1): 115.
[7]	HUANG Xiao-Chun, ZHENG Jun-Sheng, CHEN Tian-Feng, ZHANG Yi-Bo, LUO Yi, ZHENG Wen-Jie. Synthesis, Antioxidant and Anticancer Activities of 1,2,5-Selenadiazole Pyrimidine Heterocyclic Derivative ASPO [J]. Chem. J. Chinese Universities, 2012, 33(05): 976.
[8]	FAN Ying, YAO Jing, ZHOU Jian-Ping. Preparation of Nucleus-targeting Polyethyleneimine-dexamethasone Conjugates and Their Application for Gene Delivery [J]. Chem. J. Chinese Universities, 2011, 32(12): 2916.
[9]	SHI Yuan-Yuan*, LI Zhi-Qiang, GU Jing-Li, WANG Yu-Lan. Influence of Sphingomyelin Synthase Knockdown on Cell Apoptosis [J]. Chem. J. Chinese Universities, 2009, 30(9): 1759.
[10]	ZHAO Wen-Jing, NIU Feng-Lan^*. Trihydroxybenzoic Acid on Induction of the Apoptosis of Human Hepatocarcinoma SMMC-7721 Cells by Mitochondrial-dependent Pathway [J]. Chem. J. Chinese Universities, 2009, 30(2): 320.
[11]	Ablikim Kerim^*, CHEN Jia-Li, Ablimit Abdukadir. Debate on the Aromaticity of Boron- or Nitrogen-Doped Fullerene C₂₀ [J]. Chem. J. Chinese Universities, 2008, 29(9): 1810.
[12]	WANG Zhan-Feng¹, LUO Yi-Nan¹, ZHENG Yong-Hui², HAN Hai-Ling³, HONG Xin-Yu¹, JING Xia-Bin²*. Interaction of Copolymer-paclitexal Conjugate Micelles with C₆ Glioma Cells [J]. Chem. J. Chinese Universities, 2008, 29(8): 1671.
[13]	XING Yan-Xia^1,2, SU Ai³, DU Wei³, WANG Chun-Bo⁴. Polypeptide from Chlamys Farreri* Inhibits UVA-induced Apoptosis of HaCaT Cells Involving of aSMase-JNK Pathway [J]. Chem. J. Chinese Universities, 2008, 29(4): 757.
[14]	TANG Hong-Wu¹*, SMITH D. A.². Glyoxal Induced Apoptosis Studied by Near-infrared Surface-enhanced Raman Scattering [J]. Chem. J. Chinese Universities, 2008, 29(2): 254.
[15]	HUANG He-Ning^1,3, LI An-Zhang¹, WENG Lu-Na¹, LIN Qing-Mei², HUANG He-Qing^1,2*, ZHENG Zhong-Hui¹, LI Qi-Fu¹, CAI Zong-Wei⁴. Synthesis, Characterization and Binding of Tea Polyphenol-Manganese and Apoptosis Rate of Induced Tumor Cells [J]. Chem. J. Chinese Universities, 2007, 28(6): 1072.

Dynamic Observation of Cellular Localization of Fluorescein Isothiocyanate Labeled Recombinant Ganoderma Lucidum Immunoregulatory Protein(rLz-8) in NB4 APL Cell

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments