Study on Sulfated Glycosylated Fe3O4@SiO2 Nanoparticles Inducing Tumor Cells Apoptosis†

doi:10.7503/cjcu20180142

Abstract

Abstract:

Magnetic Fe₃O₄@SiO₂ nanoparticles(NPs) were first prepared through a solvothermal strategy, after that, selectively protected N-acetylglucosamine was modified on the surface of these NPs by “Click” chemistry, followed by sulfation to obtain a series of sulfated glycosylated Fe₃O₄@SiO₂ NPs with core/shell structure and various sulfation patterns. The components and morphology of these nanoparticles were characterized by X-ray diffraction(XRD), X-ray photoelectron spectroscopy(XPS) and transmission electron microscopy(TEM), respectively, before and after the modification. The effects of sulfated glycosyl groups on NPs-induced tumor cell apoptosis and protein signal regulation were studied. The results showed the prepared Fe₃O₄@SiO₂ NPs were uniform and well-dispersed. After the sulfated glycosylation, the average size of NPs increased from 110—130 nm to about 160—180 nm. These NPs could efficiently internalize into tumor cells, regulate the protein expression level of the Bcl-2/Bax pathway, and then induce cell apoptosis in a concentration-dependent manner. The activity was highly related to different sulfation patterns of glycosyl groups on the surface of NPs.

Key words: Sulfated glycosyl group, Sulfation pattern, cell apoptosis, Bcl-2/Bax pathway, Fe₃O₄@SiO₂ nanoparticles

CLC Number:

O614

TrendMD:

YUAN Jiayi, YE Baotong, WU Jing, LI Ying, CHEN Jinghua, CHEN Jingxiao. Study on Sulfated Glycosylated Fe₃O₄@SiO₂ Nanoparticles Inducing Tumor Cells Apoptosis^†[J]. Chem. J. Chinese Universities, 2018, 39(11): 2458.

Figures/Tables 11

Scheme 1 Synthetic route of a series of selectively protected N-acetylglucosamines

Fig.1 TEM images(A, B) and size distributions(C, D) of Fe3O4(A, C) and Fe3O4@SiO2(B, D) nanoparticles

Fig.2 XRD patterns of Fe3O4(a) and Fe3O4@SiO2(b)

Fig.3 XPS spectra of Fe3O4(A, B) and Fe3O4@SiO2(C, D) nanoparticles

Fig.4 TGA curves of Fe3O4@SiO2, alkynylated, and glycosylated nanoparticles(A), TEM image(B) and size distribution(C) of sulfated glycosylated nanoparticles and FTIR spectra of Fe3O4@SiO2, alkynylated, and sulfated glycosylated nanoparticles(D)

Table 1 Degree of substitution of sulfate groups on the surface of sulfated glycosylated nanoparticles

Compound	Glycosyl density/(nmol·cm^-2)	Sulfation pattern	Content of sulfate group(%)	Sulfation ratio of hydroxyl group^*(%)
1	1.35	3,4,6-S	51	63
2	0.45	6-S	28.5	69
3	0.50	3,6-S	42	64

Fig.5 XRD patterns(A) and XPS spectra(B) of sulfated glycosylated nanoparticles

Fig.6 Cell viability of Fe3O4@SiO2, sulfated N-acetylglucosamines, non-sulfated and sulfated glycosylated nanoparticles against COS7(A—C) and MGC-803(B—F) cells

Fig.7 Flow cytometric analysis(A, B) and count of MGC-803 cells(A', B') after incubating with sulfated glycosylated nanoparticles of different sulfation patterns(A, A') and 3,4,6-S nanoparticles of diffe-rent concentrations(B, B')

Fig.8 Western blot analysis of the effects of 3,4,6-sulfated glycosylated nanoparticles on Bcl-2/Bax pathway proteins of MGC-803 cellsGAPDH levels are shown as a protein loading control.

Scheme 2 Preparation of sulfated glycosylated nanoparticles(Ⅰ) and induced cancer cell apoptosis(Ⅱ)

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Study on Sulfated Glycosylated Fe₃O₄@SiO₂ Nanoparticles Inducing Tumor Cells Apoptosis^†

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