In situ Biomimetic Mineralization and Characterization of Carbon Nanotubes/chitosan Composite Microspheres†

doi:10.7503/cjcu20150346

Abstract

Abstract:

Carbon nanotubes/chitosan(CNTs/CS) composite microspheres were prepared by emulsification and cross-linking method. Nano-hydroxyapatite crystals were biomimetically formed on the surface of the composite microspheres. The influence of CNTs on the mineralization of the microspheres was investigated. The morphologies, structurs and stability properties of the microspheres before and after mineralization were studied through scanning electronic microscopy(SEM), X-ray diffraction(XRD), swelling ratio and water content tests. The results show that the formation ability of the nano-hydroxyapatite on the CNTs/CS microspheres is significantly higher than that on pure CS microspheres. And the mineralized CNTs/CS microspheres exhibit better structure stability. After culturing with MG63 cells for 7 d, the proliferation of cells cultured with mineralized CNTs/CS microspheres shows significant difference from that of the cells cultured with mineralized CS microspheres.

Key words: Carbon nanotube, Chitosan, Composite microsphere, In situ mineralization, Cellular response

CLC Number:

O611.2

TrendMD:

NIU Lulu, HUANG Di, DU Jingjing, WEI Yan, HU Chaofan, YE Jiaye, CHEN Weiyi. In situ Biomimetic Mineralization and Characterization of Carbon Nanotubes/chitosan Composite Microspheres^†[J]. Chem. J. Chinese Universities, 2015, 36(10): 1873.

Figures/Tables 9

Fig.1 SEM images of CNTs before(A) and after(B) acidification and FTIR spectrum of CNTs after treatment(C)

Fig.2 SEM images of CS microsphere(A), CNTs/CS composite microsphere(B) and the surface of CNTs/CS composite(C)

Fig.3 XRD patterns of the mineralized CS microspheres(a) and CNTs/CS microspheres(b)

Fig.4 SEM images of CS microspheres after mineralization for 3 h(A, B), 6 h(C, D) and EDS of CS microspheres after mineralization for 6 h(E)

Fig.5 SEM images of CNTs/CS microspheres(A, C) and surface of CNTs/CS(B, D) after mineralization for 3 h(A, B) and 6 h(C, D) and EDS of CNTs/CS(E)

Fig.6 SEM images of mineralized layer from CNTs/CS microspheres after sinter at 500 ℃ for 6 h with different magnifications

Table 1 Swelling ratio(Sr) and water content(Wc) of the microspheres before and after mineralization

Microsphere	S_r	W_c(%)
CS microspheres	2.525±0.967	80.5±6.3
Mineralized CS microspheres	1.656±0.624	68.6±5.1
CNTs/CS microspheres	1.094±0.443	59.8±3.5
Mineralized CNTs/CS microspheres	0.835±0.254	55.0±3.6

Fig.7 MTT assay for proliferation of MG63 cells cultured with mineralized CS microspheres and CNTs/CS composite microspheres for 1, 4 and 7 d(s), compared with the microspheres before mineralization and the blank control(tissue culture plastic) under the same culture condition

Fig.8 Formation process of n-HA crystals on the surface of CNTs/CS microspheres

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