Fabrication and Surface Properties of Hydrolyticly Function-switchable Polymer Brush†

doi:10.7503/cjcu20131092

Abstract

Abstract:

A hydrolysable cationic polycarboxybetaine ester polymer brush(PCBMA-1C2) was fabricated by photografting method with surface-coupled tertiary amine as hetergenous hydrogen donor and benzophenone(BP) as photosensitizer. The hydrolysis of PCBMA-1C2 modified surface was evaluated by studying the hydrolysis behavior of CBMA-1C2 monomer under different ammonium hydroxide concentrations. X-ray photoelectron spectroscopy(XPS) and water contact angle were used to monitor the chemical changes and hydrophilicity, respectively. The protein adsorption and platelet adhesion experiments were used to evaluate the interaction between the material surface and the biomolecules. The results show that when the concentrations of ammonium hydroxide are 0.1, 0.2, 0.3 and 0.4 mol/L, the degrees of hydrolysis(DH) of terminal ester of CBMA-1C2 monomer are 6%, 43%, 56% and almost 100%, respectively. Compared with Glass-PCBMA-1C2 surface, with the increase of DH, the protein adsorption of BSA are reduced by 3%, 76%, 93% and 96%, and that of Fg are reduced by 11%, 45%, 90% and 96%, respectively. When DH>50%, the hydrolyzed Glass-PCBMA-1C2 surface exhibits good resistance of protein adsorption and platelet adhesion.

Key words: Carboxybetaine ester, Hydrolysis, Function switch, Polymer brush, Surface property

CLC Number:

O631

TrendMD:

SHI Suqing, ZHAO Yang, ZHANG Qin, GAO Na, YANG Yang, GONG Yongkuan. Fabrication and Surface Properties of Hydrolyticly Function-switchable Polymer Brush^†[J]. Chem. J. Chinese Universities, 2014, 35(5): 1093.

Figures/Tables 9

Fig.1 1H NMR spectrum of CBMA-1C2(D2O)

Fig.2 Fabrication and hydrolysis of PCBMA-1C2 polymer brush on glass surface

Fig.3 1H NMR spectra of the hydrolyzed products of CBMA-1C2 in ammonium water of 0.1(a), 0.2(b), 0.3(c) and 0.4 mol/L(d), respectively

Fig.4 XPS spectra of glass surface before and after photografting^a. Blank glass; b. Glass-OH; c. Glass-N(CH3)2; d. Glass-PCBMA-1C2; e. Glass-PCBMA.

Table 1 Relative atomic contents(%) of glass surface

Glass	C	O	N	Si
Blank glass	12.58	58.11		29.31
Glass-OH	5.51	60.99		33.49
Glass-N(CH₃)₂	34.90	38.22	5.37	21.51
Glass-PCBMA-1C2	66.52	26.38	6.04	1.06
Glass-PCBMA	59.39	23.03	5.74	1.84

Table 2 Thickness, grafting density and degree of polymerization(DP) ofPCBMA-1C2 grafted glass surface

Sample	d/nm^a	DP^b	$M ˉ n$	σ/(Chain·nm^-2)^c
Glass-N(CH₃)₂	1.51
Glass-PCBMA-1C2	10.25	40.2	9830	0.63

Table 2 Thickness, grafting density and degree of polymerization(DP) ofPCBMA-1C2 grafted glass surface

Sample	d/nm^a	DP^b	$M ˉ n$	σ/(Chain·nm^-2)^c
Glass-N(CH₃)₂	1.51
Glass-PCBMA-1C2	10.25	40.2	9830	0.63

Fig.5 Static water contact angle for the glass surface^(A) Blank glass; (B) Glass-OH; (C) Glass-N(CH3)2; (D) Glass-PCBMA-1C2; (E) Glass-A-PCBMA-1C2;(F) Glass-B-PCBMA-1C2; (G) Glass-C-PCBMA-1C2; (H) Glass-PCBMA.

Fig.6 Protein adsorption for the glass surface^ Blank glass; Glass-PCBMA-1C2; Glass-A-PCBMA-1C2;

Fig.7 Platelet adhesion for the glass surface^(A) Blank glass; (B) Glass-PCBMA-1C2; (C) Glass-A-PCBMA-1C2; (D) Glass-B-PCBMA-1C2;(E) Glass-C-PCBMA-1C2; (F) Glass-PCBMA.

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