Effects of Ionic Liquids with Different Anion Composition on Cell Growth, Metabolism and Catalysis Characteristics of Penicillium purpurogenum Li-3†

doi:10.7503/cjcu20130859

Abstract

Abstract:

The wild-type Penicillium purpurogenum Li-3 strain is capable of expressing highly β-glucuronidase, a intracellular enzyme with excellent ability to biotransform glycyrrhizin(GL) into glycyrrhetinic acid monoglucuronide(GAMG) possessing best biological function. To improve the production of GAMG, the effects of ionic liquids with different anion composition on Penicillium purpurogenum Li-3 cells properties of growth, metabolism, permeability of cell membrane and catalysis were studied in this paper. The results showed that [Bmim]Ac and [Bmim]Tf₂N had obvious inhibitory effects on cell growth and metabolism. Six kinds of ionic liquids all improved the permeability of cell membrane, and [Bmim]Ac was the most remarkable. In the two catalytic medium respectively containing 25% hydrophobic [Bmim]PF₆ and [Bmim]Tf₂N, conversion rates of substrate GL were 82.73% and 89.84%, and the yields of GAMG were 68.65% and 76.23%, respectively. Thus, the substrate GL conversion increased slightly, but the yield of GAMG were about two times higher than that in the pure buffer solution without ionic liquids(conversion rate of GL 81.82%, yield of GAMG 33.31%). It was obvious that hydrophobic [Bmim]PF₆ and [Bmim]Tf₂N were capable of promoting the selectivity of the whole cell catalysis. While hydrophilic [Bmim]Ac, [Bmim]Cl and [Bmim]NO₃ obviously had inhibition in different degrees on the cell’s catalytic activity. These results indicated that the anions in ionic liquids played a crucial role as biological catalytic medium.

Key words: Ionic liquid, Anion, Penicillium purpurogenum Li-3, Whole cell, Catalytic property

CLC Number:

TrendMD:

LI Yang, CAO Hong, OUYANG Qiaofeng, LI Chun, ZHENG Lanlan, ZHANG Xin. Effects of Ionic Liquids with Different Anion Composition on Cell Growth, Metabolism and Catalysis Characteristics of Penicillium purpurogenum Li-3^†[J]. Chem. J. Chinese Universities, 2014, 35(5): 1057.

Figures/Tables 4

Fig.1 Influence of ionic liquids on the cell biomass^a. Aqueous; b. [Bmim]Cl; c. [Bmim]PF6; d. [Bmim]BF4; e. [Bmim]NO3; f. [Bmim]Ac; g. [Bmim]Tf2N.

Fig.2 Metabolic activity retention of Penicillium purpurogenum Li-3 cell in different ionic liquids^a. Aqueous; b. [Bmim]Cl; c. [Bmim]PF6; d. [Bmim]BF4; e. [Bmim]NO3; f. [Bmim]AC; g. [Bmim]Tf2N.

Fig.3 Strain cell membrane permeability to proteins(A) and RNA(B) in different ionic liquids(25%, volume fraction)^★ Aqueous; ■ [Bmim]PF6; ● [Bmim]BF4; □ [Bmim]NO3; ▼ [Bmim]Cl; ○ [Bmim]Ac; ▽ [Bmim]Tf2N.

Fig.4 Reaction progress curves of Penicillium purpurogenum Li-3 cell in different volume fractions of ionic liquids^ (A) [Bmim]PF6; (B) [Bmim]PF6; (C) [Bmim]PF6; (D) [Bmim]PF6; (E) [Bmim]PF6. ★ Aqueous; volume fraction: ○ 6%; ▼ 10%; □ 14%; ◆ 20%; ▽ 25%.

References 23

[1]	Feng S. J., Li C., Xu X. L., Wang X. Y., J. Mol. Catal. B-Enzym., 2006, 43, 63—67
[2]	Feng S. J., Li C., Li H.,Wang X. Y., J. Chem. Eng. Chin. Univ., 2007, 21(6), 977—982
	(冯世江, 李春, 李晖, 王小艳.高校化学工程学报, 2007,21(6), 977—982)
[3]	Zou S. P., Zhang Q., Li L. M., Guo Y. X., Dai D. Z., Li C., CIESC Journal, 2009, 60(8), 2040—2045
	(邹树平, 张琦, 李丽敏, 郭燕霞, 戴大章, 李春.化工学报, 2009,60(8), 2040—2045)
[4]	Pfruender H., Amidjoin M., Kragl U., Weuster-Botz D., Angew. Chem. Int. Ed., 2004, 43(34), 4529—4531
[5]	Pfruender H., Jones R., Weuster-Botz D., J. Biotechnol., 2006, 124(1),182—190
[6]	Zhang F., Ni Y., Sun Z. H., Zheng P., Lin W. Q., Zhu P., Ju N. F., Chin. J. Catal., 2008, 29(6), 577—582
	(张帆, 倪晔, 孙志浩, 郑璞, 林文清, 朱坡, 居年丰.催化学报, 2008,29(6), 577—582)
[7]	He D. M., Imdad K., Dai D. Z., Li C., Process Biochem., 2010, 45(12), 1916—1922
[8]	Cao H., Sun Q., Duan H. Y., Ye H., Chen S. Y., Liu G. Y., J. Beijing Insti. Technol., 2011, 31(6), 732—736
	(曹红, 孙倩, 段海燕, 叶海, 陈思羽, 刘桂艳.北京理工大学学报, 2011,31(6), 732—736)
[9]	Chen J. Y., Zou S. P., He D. M., Yang X. G., Li C., CIESC Journal, 2011, 62(11), 3136—3141
	(陈金燕, 邹树平, 何冬梅, 杨晓刚, 李春.化工学报, 2011,62(11), 3136—3141)
[10]	Cao H., Sun Q., Li C., Li K. X., Li J., Chem. J. Chinese Universities, 2013, 34(8), 1873—1879
	(曹红, 孙倩, 李春, 李开雄, 李军.高等学校化学学报, 2013,34(8), 1873—1879)
[11]	Wang X. P., Xing S. L., Tianjin Chem. Ind., 2009, 23(3), 40—41
	(王孝平, 邢树礼.天津化工, 2009,23(3), 40—41)
[12]	Luan Y.S., Bao Y. M., Handbook of Biochemical Experiment Techniques, Chemical Industry Press, Beijing, 2005, 18—19
	(栾雨时, 包永明. 生物工程实验技术手册, 北京: 化学工业出版社, 2005, 18—19)
[13]	Ma G. L., Zhang X. J., Jin L., China Brewing, 2009, 207(6), 22—24
	(马歌丽, 张学军, 靳丽.中国酿造, 2009,207(6), 22—24)
[14]	Chen X. Q., Chen S., Yuan X. Z., J. Basic Sci. Engin., 2009, 17(5), 697—706
	(陈晓青, 陈石, 袁兴中.应用基础与工程科学学报, 2009,17(5), 697—706)
[15]	Yang Z., Liu X. J., Chen C., Peter J. H., BBA-Proteins Proteom., 2010, 1804(4), 821—828
[16]	Yang Z., J. Biotechnol., 2009, 144(1), 12—22
[17]	Wang W., Zong M. H., Lou W. Y., Chin. J. Catal., 2011, 32(6), 1003—1010
	(汪薇, 宗敏华, 娄文勇.催化学报, 2011,32(6), 1003—1010)
[18]	Chen Z. G., Zong M. H., Gu Z. X., Chin. J. Org. Chem., 2009, 29(5), 672—680
	(陈志刚, 宗敏华, 顾振新.有机化学, 2009,29(5), 672—680)
[19]	Robert J. C., Catherine L.W., Stephanie S., Royston G., Gill S., Green Chem., 2008, 10, 685—691
[20]	Gu Y. L., Peng J. J., Qiao K., Yang H. Z., Shi F., Deng Y. Q., Prog. Chem., 2003, 15(3), 222—241
	(顾彦龙, 彭家建, 乔琨, 杨宏洲, 石峰, 邓友全.化学进展, 2003,15(3), 222—241)
[21]	Zhao H., J. Mol. Catal. B, 2005, 37, 16—25
[22]	Lou W. Y., Zong M. H., Chem. J. Chinese Universities, 2007, 28(7), 1283—1287
	(娄文勇, 宗敏华.高等学校化学学报, 2007,28(7), 1283—1287)
[23]	Zhao H., Lee J., Song Z.Y., Olarongbe O., Tetrahedron: Asymmetry, 2006, 17, 2491—2498