Chem. J. Chinese Universities ›› 2019, Vol. 40 ›› Issue (1): 160.doi: 10.7503/cjcu20180367
• Polymer Chemistry • Previous Articles Next Articles
ZHANG Pan1, SUN Pengfei2, LIN Mingchang1, GAO Zhenfei1, CHEN Guosong1,*()
Received:
2018-05-17
Online:
2019-10-15
Published:
2019-08-21
Contact:
CHEN Guosong
E-mail:guosong@fudan.edu.cn
Supported by:
CLC Number:
TrendMD:
ZHANG Pan,SUN Pengfei,LIN Mingchang,GAO Zhenfei,CHEN Guosong. Kinetic Study of the Interaction Between Lactose-containing Assembly and Protein Through Dynamic Light Scattering Technique†[J]. Chem. J. Chinese Universities, 2019, 40(1): 160.
Sample | PGlc(shell) | PLac(shell) | PtBMA(core) |
---|---|---|---|
Lac-0 | 100 | 0 | 100 |
Lac-25 | 75 | 25 | 100 |
Lac-50 | 50 | 50 | 100 |
Lac-75 | 25 | 75 | 100 |
Lac-100 | 0 | 100 | 100 |
Table 1 Mass fraction(%) of the shell and core of glyco-micelles
Sample | PGlc(shell) | PLac(shell) | PtBMA(core) |
---|---|---|---|
Lac-0 | 100 | 0 | 100 |
Lac-25 | 75 | 25 | 100 |
Lac-50 | 50 | 50 | 100 |
Lac-75 | 25 | 75 | 100 |
Lac-100 | 0 | 100 | 100 |
Sample | <Rh>/nm | PDI |
---|---|---|
Lac-0 | 63 | 0.08 |
Lac-25 | 60 | 0.22 |
Lac-50 | 72 | 0.28 |
Lac-75 | 80 | 0.38 |
Lac-100 | 60 | 0.10 |
Table 2 Hydrodynamic radius of glyco-micelles with different coverage degree of lactose*
Sample | <Rh>/nm | PDI |
---|---|---|
Lac-0 | 63 | 0.08 |
Lac-25 | 60 | 0.22 |
Lac-50 | 72 | 0.28 |
Lac-75 | 80 | 0.38 |
Lac-100 | 60 | 0.10 |
Fig.3 <Rh> of Lac-100 and Lac-0 with titration of SBA(A) and <Rh> distribution of Lac-100 with titration of SBA(B)Sidenotes are the volume of SBA added to the solution. Mean value of three continuous experiments. Concentration of glyco-micelles is 0.04 mg/mL.
Fig.5 Crystal structure of SBA complexed with 2,6-pentasaccharide(PDB code 2SBA)(A) and pseudomonas aeruginosa lectin(LecA) complexed with GalA-QRS(PDB code 4LKD)(B)
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