Kinetics Study of Chiral Recognition Between Protein and Amino Acid Enantiomers by Surface Plasmon Resonance

Chem. J. Chinese Universities ›› 2011, Vol. 32 ›› Issue (8): 1709.

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Kinetics Study of Chiral Recognition Between Protein and Amino Acid Enantiomers by Surface Plasmon Resonance

SHEN Gang-Yi^1*, GAO Yan¹, DAI Dong-Sheng^2*, CUI Jian¹, LIU Yu-Ming¹, PEI Ling-Peng¹

1. Key Laboratory of Chinese Minority Traditional Medicine, State Ethnic Affairs Commission and Ministry of Education of China, Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, China;
2. Yabao Pharmaceutical Group Co. Ltd., Taiyuan 044602, China

Received:2011-01-21 Revised:2011-05-19 Online:2011-08-10 Published:2011-07-19
Contact: SHEN Gang-Yi;DAI Dong-Sheng E-mail:sgy@iccas.ac.cn;dongshengdai@126.com
Supported by:
国家自然科学基金(批准号: 81001595)、中央高校基本科研业务费专项资金中央民族大学自主科研项目(批准号: 0910KYQN67)和新疆名医名方与特色方剂学实验室开放课题(批准号: XJDX0910-2010-01)资助.

Abstract

Abstract: Based on surface plasmon resonance technique, a new method has been developed for efficiently recognizing the chiral discrimination between the small molecule L- and D-amino acid enantiomers without any labeling. Bovine serum albumin and lysozyme were selected as chiral recognition probes, and phenylalanine and tryptophan including L-, D- four enantiomers were used as analytes. While six different concentrations of each amino acid enantiomers interacted with two proteins probes at the same time, the binding kinetics informations of them were obtained in real time. The results showed that proteins had excellent chiral recognition capability to L- and D- enantiomers. The chiral discrimination between each of amino acid enantiomers could be recognized obviously by comparison either their binding kinetics parameters or sensorgram. The results also indicated that the affinity of protein and L-amino acid was bigger than that of protein and D-amino acid.

Key words: Suface plasmon resonance(SPR), Chiral recognition, Amino acid, Kinetics

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SHEN Gang-Yi*, GAO Yan, DAI Dong-Sheng*, CUI Jian, LIU Yu-Ming, PEI Ling-Peng. Kinetics Study of Chiral Recognition Between Protein and Amino Acid Enantiomers by Surface Plasmon Resonance[J]. Chem. J. Chinese Universities, 2011, 32(8): 1709.

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Kinetics Study of Chiral Recognition Between Protein and Amino Acid Enantiomers by Surface Plasmon Resonance

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