Preparation of Glycyrrhetinic Acid-modified PEG-PLGA Nanoparticles and the Affinity Evaluation on Hepatoma Cells

Chem. J. Chinese Universities ›› 2011, Vol. 32 ›› Issue (2): 416.

• Articles • Previous Articles

Preparation of Glycyrrhetinic Acid-modified PEG-PLGA Nanoparticles and the Affinity Evaluation on Hepatoma Cells

HUANG Wei, WANG Ping, WANG Wei, ZHANG Yue, ZHANG Chuang-Nian, TIAN Qin, WANG Xiu-Hua, LIU Yuan, YUAN Zhi*

Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, Nankai University, Tianjin 300071, China

Received:2010-03-16 Revised:2010-04-22 Online:2010-02-10 Published:2011-02-23
Contact: YUAN Zhi E-mail:zhiy@nankai.edu.cn
Supported by:
国家自然科学基金(批准号: 50873048)和天津市自然科学基金(批准号: 07JCZDJC00700) 资助.

Abstract

Abstract: In this study, glycyrrhetinic acid-modified PEG-PLGA copolymer was fabricated, and was formed into nanoparticles (NPs) via solvent evaporation method with D-α-tocopheryl poly(ethylene glycol) succinate (TPGS) as a stabilizer. The physicochemical properties of the present system were investigated by NMR, IR, DLS, Ztea and TEM measurements. The cytotoxicity against hepatoma cells was studied based on MTT assay, and the affinity between the cells and the NPs were preliminarily evaluated by fluorescence microscopy. The results showed that the NPs were regularly spherical in shape with a hydrodynamic diameter around 128.2 nm, and the ztea potential of the NPs was about -16.2 mv. Besides, the NPs exhibited good stability against electrolyte solution. MTT result suggested that the NPs almost had no cytotoxicity on cells. Furthermore, the in vitro cell uptake result indicated that GA-modified NPs had a high affinity with hepatoma cells, and could be a promising liver targeted material.

Key words: Glycyrrhetinic acid, PEG-PLGA, D-α-tocopheryl poly(ethylene glycol) succinate (TPGS), Liver targeted material.

TrendMD:

HUANG Wei, WANG Ping, WANG Wei, ZHANG Yue, ZHANG Chuang-Nian, TIAN Qin, WANG Xiu-Hua, LIU Yuan, YUAN Zhi*. Preparation of Glycyrrhetinic Acid-modified PEG-PLGA Nanoparticles and the Affinity Evaluation on Hepatoma Cells[J]. Chem. J. Chinese Universities, 2011, 32(2): 416.

[1]	YUAN Baoming,DONG Xiaoming,YANG Fan,PENG Chuangang,WANG Jincheng,Wu Dankai. Preparation and the Antibacterial Activity of AgNPs/PLGA-PEG-PLGA Composite Hydrogel ^† [J]. Chem. J. Chinese Universities, 2019, 40(10): 2225.
[2]	DONG Xiaoming, YUAN Baoming, CHEN Bingpeng, HE Chaoliang, WANG Jincheng, CHEN Xuesi. In vitro Antibacterial Activity of PLGA-PEG-PLGA Thermosensitive Hydrogels Loaded with Vancomycin [J]. Chem. J. Chinese Universities, 2017, 38(5): 866.
[3]	YANG Meiyue, WANG Wei, TIAN Zhiqing, LI Yingying, YUAN Zhi. Preparation of Glycyrrhetinic Acid-modified Sodium Alginate Microgel Spheres for 3D Cell Culture^† [J]. Chem. J. Chinese Universities, 2017, 38(2): 326.
[4]	Zhang Xin, LI Yang, CAO Hong, Ouyang Qiaofeng, Zheng Lanlan, LI Chun. Effect of the Ionic Liquids with Anion Tf₂N^- on the Whole Cell Catalytic Properties of Penicillium Purpurogenum Li-3 Strain^† [J]. Chem. J. Chinese Universities, 2016, 37(10): 1870.
[5]	GUO Hua, YANG Chengling, WANG Wei, LAI Quanyong, YUAN Zhi. Preparation of Liver-targeted Nano-prodrug Based on Sodium Alginate Derivative and the Study on Antitumor Activity^† [J]. Chem. J. Chinese Universities, 2014, 35(8): 1835.
[6]	CAO Hong, SUN Qian, LI Chun, LI Kai-Xiong, LI Jun. Effect of Hydrophilic Ionic Liquid [EMIM]BF₄ on the Catalytic Activity of Immobilized β-Glucuronidase [J]. Chem. J. Chinese Universities, 2013, 34(8): 1873.
[7]	WANG Yan, WANG Xiao-Ying, LV Yan-Yun, XU Wen-Bin, GUO Yi, XU Li. Preparation of Magnetic Drug Carriers with Evodiamine and Fe₃O₄ Nanoparticles Co-encapsulated [J]. Chem. J. Chinese Universities, 2013, 34(12): 2866.
[8]	XU Chong-En, YANG Jing, LUO Bing-Hong, LI Jian-Hua, XU Wan, ZHOU Chang-Ren. Synthesis and Characterization of PLGA-PEG-PLGA Triblock Copolymer with Side Propargyl Groups [J]. Chem. J. Chinese Universities, 2012, 33(10): 2138.
[9]	GAO Zhen-Bei, HU Jun, KANG Xiao, XU Chuan-Lian, JU Yong. Synthesis of A-Ring Functional Derivatives of 18β-Glycyrrhetinic Acid and Their Antiproliferative Effect in Tumor Cells [J]. Chem. J. Chinese Universities, 2012, 33(04): 750.
[10]	HU Jun, WU Yang, ZHAO Chang-Qi, JU Yong*. Synthesis and Anti-tumor Activity of Opened A-Ring Modified 18β-Glycyrrhetinic Acid Derivatives [J]. Chem. J. Chinese Universities, 2010, 31(9): 1762.
[11]	ZHA Rui-Tao^1,2, HE Xiao-Ting¹, DU Tian¹, YUAN Zhi¹*. Synthesis and Characterization of Chitosan Nanoparticles Modified by Glycyrrhetinic Acid as a Liver Targeting Drug Carrier [J]. Chem. J. Chinese Universities, 2007, 28(6): 1098.
[12]	LIU Li-Jun², YONG Jian-Ping², DAI Xiao-Jun², JIA Jiong¹, WANG Xi-Zhao¹, WANG Jian-Wu¹ . Synthesis of Novel Isoxazole Contained Glycyrrhetinic Acid Derivatives [J]. Chem. J. Chinese Universities, 2006, 27(9): 1669.
[13]	CHA RuiTao, DU Tian, YUAN Zhi*. Syntheses and Characterization of Poly(γ-benzyl-L-glutamate) Containing Liver Targeting Group in Main Chain [J]. Chem. J. Chinese Universities, 2006, 27(5): 885.

Preparation of Glycyrrhetinic Acid-modified PEG-PLGA Nanoparticles and the Affinity Evaluation on Hepatoma Cells

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 13

Recommended Articles

Metrics

Comments