CJCU

Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (9): 1729.

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Screening and Cluster of Differentially Expressed Gene of MSCs Induced by Shuanglong Prescription Components

FAN Xue-Mei1, LI Xue2, LIANG Qiong-Lin3, HU Ping4, WANG Yi-Ming3, LUO Guo-An1,3*   

  1. 1. School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China;
    2. School of Medicine,
    3. Department of Chemistry, Tsinghua University, Beijing 100084, China;
    4. School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China
  • Received:2008-09-12 Online:2009-09-10 Published:2009-09-10
  • Contact: LUO Guo-An. E-mail: luoga@tsinghua.edu.cn
  • Supported by:

    科学技术部“十一五”科技支撑计划(批准号: 2006BA108B04-01)和国家“九七三”计划(批准号: 2005CB523503)资助.

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Abstract:

To investigate the effects of Shuanglong prescription components and the differential genes on the stem cells differentiation into cardiomyocytes via cDNA microarray, the differentially expressed genes were recorded by cluster analysis. We divided and separately cultured the Rat BMSCs, collected the cell samples on 10, 20, 30 and 40 d, then distilled total RNA. Microarray was used to detect the differentially expressed genes between each group. The differential expressed genes were screened and analysed by cluster. We got 179 differentially expressed genes during the differentiation. By bioinformal analysis, these genes were associated with metabolism, signal transduction etc. Using hierarchical method, we found that 10 and 20 d samples were clustered in one class, 30 and 40 d samples were clustered in another class. It illuminated that BMSCs after induced may generated distinctly change between 20 and 30 d. Shuanglong prescription components may induced BMSCs differentiated into cardiomyocyte-like cells.

Key words: Shuanglong prescription; Gene chip; Differentially expressed gene; Cluster analysis

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