Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (9): 1733.

• Articles • Previous Articles     Next Articles

Interaction Between Water-soluble Hydroxylated Single-wall Carbon Nanotubes and Human Serum Albumin

LIU Shu-Fang, YIN Jun-Fa, SONG Mao-Yong, WANG Hai-Lin*   

  1. State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 10085, China
  • Received:2008-09-01 Online:2009-09-10 Published:2009-09-10
  • Contact: WANG Hai-Lin. E-mail: hlwang@rcees.ac.cn
  • Supported by:

    中国科学院知识创新工程青年人才领域前沿项目(批准号: RCEES-QN-200715)资助.

Abstract:

The interaction between water-soluble hydroxylated single-wall carbon nanotube and human serum albumin and other related effects were investigated using fluorescence spectroscopy, absorption spectroscopy, synchronous fluorescence spectroscopy and transmission electron microscopy(TEM) methods. Fluorescence quenching of human serum albumin by a hydroxylated single-wall carbon nanotube was observed, indicating the interaction between them occurs. TEM images show the hydroxylated carbon nanotubes system can be stabilized and dispersed by the absorption of human serum albumin on the surface of the hydroxylated single-wall carbon nanotubes. The synchronous fluorescence spectra of human serum albumin indicate the slight change of HSA conformation and the microenvironment of tryptophan residues in human serum albumin. The experimental results may provide us some important information for the understanding of transportation of hydroxylated single-wall carbon nanotubes and other related biological effects.

Key words: Carbon nanotubes; Human serum albumin; Transmission electron microscopy(TEM); Fluorescence; Synchronous fluorescence; Fluorescence quenching

TrendMD: