Anodic Stripping Voltammetric Immunoassay Based on Coupling Copper-enhanced Nanogold Tags with Magnetic Separation for Highly Sensitive Detection of Proteins

Abstract

Abstract:

A novel and highly sensitive anodic stripping voltammetric immunoassay was proposed on the basis of chemical copper-enhanced gold nanoparticle(nanogold) tags in vitro as the amplification approach with the aid of magnetic separation technique. In a homogeneous bulk solution phase, the human IgG(hIgG) as a model of test system was captured by goat anti-human IgG antibody(anti-hIgG)-conjugated SiO₂@Fe₃O₄ magnetic nanoparticle and anti-hIgG-conjugated nanogold, followed by the copper metal dissolution in diluted nitric acid solution for anodic stripping voltammetry measurement. Comparing with the traditional solid interface reaction-based metal immunoassay, this novel one which introduce homogeneous reaction strategy combined with magnetic separation can provide some advantages, including easy of use, shorter analytical time, and especially amplified capability of detecting hIgG. It can quantitatively determine hIgG in the concentration range of 0.1—1000 ng/mL, with a detection limit about 73 pg/mL.

Key words: Nanogold, Copper-enhanced amplification, Magnetic separation, Metal immunoassay

TrendMD:

SHEN Guang-Yu*, FENG Hui, HAO Ai-Ping. Anodic Stripping Voltammetric Immunoassay Based on Coupling Copper-enhanced Nanogold Tags with Magnetic Separation for Highly Sensitive Detection of Proteins[J]. Chem. J. Chinese Universities, 2009, 30(5): 886.

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