Preparation and Biological Characterization of Antibody and DNA Dual-labeled Gold Nanoparticles

Chem. J. Chinese Universities

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Preparation and Biological Characterization of Antibody and DNA Dual-labeled Gold Nanoparticles

LI Fu-Rong^1*, QIAO Fei-Yan², KONG Xiao-Li¹, ZHOU Han-Xin¹, QI Hui¹, REN Li-Li¹

1. Clinic Medical Research Center, The 2nd Clinic Medical College(Shenzhen People’s Hospital), Jinan University, Shenzhen 518020, China;
2. Department of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China

Received:2008-04-23 Revised:1900-01-01 Online:2009-01-10 Published:2009-01-10
Contact: LI Fu-Rong

Abstract

Abstract: Gold nanoparticles labeled by both antibody(IgG) and single stranded DNA(ss-DNA) were synthesized and characterized. The stability and reactivity of the dual-labeled nanoparticles were compared with the conventional IgG or ss-DNA modified nanoparticles. It was found that the IgG adsorption significantly improved the stability of the nanoparticles in aqueous solution, which is beneficial for attaching ss-DNA. The presence of IgG also effectively prohibits the desorption of ss-DNA against dithiothreitol(DTT) displacement. The coverage on dual-labeled nanoparticles was found to be (50±15) ss-DNA/nanoparticle and (10±2) IgG/nanoparticle, respectively, compared to the value of (70±15) ss-DNA/nanoparticle of only ss-DNA-labeled gold nanoparticles. Dot-immuno and cross-linking experiments confirmed that both the IgG and ss-DNA retained their bioactivity on the nanoparticle surface. The dual-labeled nanoparticles have potential to be used as novel bio-probes for ultrasensitive detection.

Key words: Gold nanoparticle, Bio-probes, DNA, Antibody

CLC Number:

O629.7

TrendMD:

LI Fu-Rong^1*, QIAO Fei-Yan², KONG Xiao-Li¹, ZHOU Han-Xin¹, QI Hui¹, REN Li-Li¹. Preparation and Biological Characterization of Antibody and DNA Dual-labeled Gold Nanoparticles[J]. Chem. J. Chinese Universities, doi: .

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Preparation and Biological Characterization of Antibody and DNA Dual-labeled Gold Nanoparticles

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