Oxidative DNA Cleavage Catalyzed by Mn(Ⅲ) Corroles

Chem. J. Chinese Universities

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Oxidative DNA Cleavage Catalyzed by Mn(Ⅲ) Corroles

LIU Hai-Yang^1,2*, LIU Lan-Ying¹, ZHANG Lei³, YING Xiao⁴, WANG Xiang-Li¹, JIANG Huan-Feng¹, CHANG Chi-Kwong²*

1. Department of Chemistry, South China University of Technology, Guangzou 510641, China;
2. Department of Chemistry, The Hong Kong University of Science and Technology, Hong Kong, China;
3. Department of Pharmaceutical Engineering,
4. Department of Applied Physics, South China University of Technology, Guangzou 510641, China

Received:2007-05-08 Revised:1900-01-01 Online:2007-09-10 Published:2007-09-10
Contact: LIU Hai-Yang

Abstract

Abstract: Several Mn(Ⅲ) 10-(hydroxylphenyl)-5,15-bis(pentafluoro-phenyl)corroles with the hydroxyl at ortho-, meta- and para-position of 10-phenyl group were used as the catalysts in the oxidative cleavage of plasmid DNA in the presence of H₂O₂. The catalytic system was composed of 10 μL 0.5 mg/mL pC DNA 6 in TE buffer solution(pH=8.0, 4.0×10^-2 mol/L Tris, 1.0×10^-3 mol/L EDTA), 10 μL 1.0×10^-5 mol/L Mn(Ⅲ) corrole in DMSO and 10 μL 0.3% H₂O₂. After 8 h incubation at room temperature, significant oxidative DNA damage could be observed with the ortho-hydroxyl Mn(Ⅲ) corrole showing a better activity as detected by agarose-gel electrophoresis. Plasmid DNA was completely damaged after 12 h incubation. These observations show Mn(Ⅲ) corrole can be used as an artificial model of nuclease.

Key words: Manganese, Corrole, DNA, Oxidative cleavage

CLC Number:

O611.3

TrendMD:

LIU Hai-Yang^1,2*, LIU Lan-Ying¹, ZHANG Lei³, YING Xiao⁴, WANG Xiang-Li¹, JIANG Huan-Feng¹, CHANG Chi-Kwong²*. Oxidative DNA Cleavage Catalyzed by Mn(Ⅲ) Corroles[J]. Chem. J. Chinese Universities, doi: .

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Oxidative DNA Cleavage Catalyzed by Mn(Ⅲ) Corroles

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