Chem. J. Chinese Universities

• 研究论文 • Previous Articles     Next Articles

Fluorescence Resonance Energy Transfer Between CdTe Quantum Dot Donors and Au Nanoparticles Labeled DNA Acceptors

ZHANG Ji-Mei1, DAI Zhao1, GUO Ning1, XU Shi-Chao1, DONG Quan-Xi1, SUN Bo2   

    1. School of Material Science and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300160;
    2. Chemistry School, Nankai University,Tianjin 300072,China
  • Received:2006-02-22 Revised:1900-01-01 Online:2007-02-10 Published:2007-02-10
  • Contact: ZHANG Ji-Mei

Abstract: CdTe semiconductor nanoparticles which were synthesized in water phase were taken as the energy donors. CdTe nanoparticles were linked to 5'-NH2-DNA by Schiff's reaction. Au nanoparticles which were prepared by sodium citrate reduction of chloroauric acids were taken as the energy acceptors. Au nanoparticles were linked to 3'-SH—DNA by Au—S bond. The fluoresscence resonance energy transfer(FRET) probes are generated through hybridization of complementary DNA bound to the QDs and Au nanoparticles. The fluorescence spectra of CdTe-DNA, CdTe-DNA-Au and CdTe-DNA-Au+target DNA were measured . The data show that the fluorescence intensity of CdTe-DNA-Au conjugates was the weakest. When adding a complementary ssDNA to CdTe-DNA-Au conjugates, the fluorescence intensity of the system increased. This result shows that the configuration of FRET was successful.

Key words: Au nanoparticle, CdTe nanoparticle, Fluorescence resonance energy transfer(FRET), Fluorescence quenching

CLC Number: 

TrendMD: