Effects of the Dimerization of [Ru(Phen)2dppz]2+ on the Calf Thymus Binding Properties

Chem. J. Chinese Universities

• 研究简报 • Previous Articles Next Articles

Effects of the Dimerization of [Ru(Phen)₂dppz]²⁺ on the Calf Thymus Binding Properties

NIE Jing, HAN Mei-Jiao, WANG Ke-Zhi*

College of Chemistry, Beijing Normal University, Beijing 100875, China

Received:2007-04-23 Revised:1900-01-01 Online:2007-10-10 Published:2007-10-10
Contact: WANG Ke-Zhi

Abstract

Abstract: The dimerization of a well-known DNA light-switch [Ru(phen)₂dppz]²⁺(phen=1,10-phenanthroline, dppz=dipyrido[3,2-a:2',3'-c]phenazine), and its effect on calf thymus binding properties were studied by UV-Vis and emission spectroscopy in Tris-HCl buffered 50 mmol/L NaCl(pH=7.1). The results show that the complex has a strong tendency to dimerize with a dimerization constant of (1.8±0.3)×10⁶ L/mol at the same order of magnitude as the DNA binding constant of (6.0±1.2)×10⁶ L/mol for the Ru(Ⅱ) complex dimer, but one order of magnitude less than (4.9±1.5)×10⁷ L/mol for the Ru(Ⅱ) complex monomer. This study has provided insight into a fundamental but important issue that should be taken into account for the DNA binding studies.

Key words: Ruthenium, Phenanthroline, DNA, Light switch, Dimerization

CLC Number:

O614
O641.3

TrendMD:

NIE Jing, HAN Mei-Jiao, WANG Ke-Zhi*. Effects of the Dimerization of [Ru(Phen)₂dppz]²⁺ on the Calf Thymus Binding Properties[J]. Chem. J. Chinese Universities, doi: .

[1]	LIU Suyu, DING Fei, LI Qian, FAN Chunhai, FENG Jing. Azobenzene-integrated DNA Nanomachine [J]. Chem. J. Chinese Universities, 2022, 43(8): 20220122.
[2]	WU Yushuai, SHANG Yingxu, JIANG Qiao, DING Baoquan. Research Progress of Controllable Self-assembled DNA Origami Structure as Drug Carrier [J]. Chem. J. Chinese Universities, 2022, 43(8): 20220179.
[3]	WANG Junyang, LIU Zheng, ZHANG Qian, SUN Chunyan, LI Hongxia. Application of DNA Silver Nanoclusters in the Fluorescence Biosensors based on Functional Nucleic Acids [J]. Chem. J. Chinese Universities, 2022, 43(6): 20220010.
[4]	WU Yangyi, CHEN Jianping, Ai Yijing, WANG Qingxiang, GAO Fei, GAO Feng. Synthesis of 2-（2-Hydroxy-3-methoxyphenyl）-C₆₀ and Its Application for Sensing of Cauliflower Mosaic Virus 35S Promotor [J]. Chem. J. Chinese Universities, 2021, 42(6): 1754.
[5]	YANG Xinjie, LAI Yanqiong, LI Qiuyang, ZHANG Yanli, WANG Hongbin, PANG Pengfei, YANG Wenrong. An Enzyme-free and Label-free Fluorescent Probe for Detection of Microcystin-LR Based on Circular DNA-Silver Nanoclusters [J]. Chem. J. Chinese Universities, 2021, 42(12): 3600.
[6]	DUAN Jun, ZHOU Pingping, ZHANG Liqian, MA Lin, YU Wen, ZHU Meiqi, ZHUANG Minyan, YANG Fenglei, CAO Changsheng, ZHANG Peng, SHI Yanhui. Syntheses of Imidazole-coordinated Ru（II） Half-sandwich Macrocyclic Complexes and Their Anti-cancer Activity [J]. Chem. J. Chinese Universities, 2021, 42(12): 3589.
[7]	LIU Xuejiao, YANG Fan, LIU Shuang, ZHANG Chunjuan, LIU Qiaoling. Progress in Aptamer-targeted Membrane Protein Recognition and Functional Regulation [J]. Chem. J. Chinese Universities, 2021, 42(11): 3277.
[8]	MAO Yu, QU Hao, ZHENG Lei. Research Progress on RNA⁃cleaving DNAzyme for the Detection of Pathogenic Bacteria [J]. Chem. J. Chinese Universities, 2021, 42(11): 3445.
[9]	HU Ling, YIN Yao, KE Guoliang, ZHANG Xiaobing. Regulation of Cell-cell Interactions Based on DNA Nanostructures [J]. Chem. J. Chinese Universities, 2021, 42(11): 3284.
[10]	PENG Huo, GAO Zehang, LIAO Chengyue, WANG Xiaodong, ZHOU Hongbo, ZHAO Jianlong. Robust Droplet Digital PCR Chip for Absolute Quantitative Detection of Nucleic Acid [J]. Chem. J. Chinese Universities, 2020, 41(8): 1760.
[11]	REN Yushuang, GUO Yuanyuan, LIU Xueyi, SONG Jie, ZHANG Chuan. Platinum(Ⅳ) Prodrug-grafted Phosphorothioate DNA and Its Self-assembled Nanostructure for Targeted Drug Delivery [J]. Chem. J. Chinese Universities, 2020, 41(8): 1721.
[12]	YAN Lei, MAO Xiuhai, ZUO Xiaolei. Biomimicry of Cellular Membrane with Framework Nucleic Acids^† [J]. Chem. J. Chinese Universities, 2020, 41(7): 1415.
[13]	ZHANG Kaixiang, LIU Junjie, SONG Qiaoli, WANG Danyu, SHI Jinjin, ZHANG Haiyue, LI Jinghong. Multifunctional DNA Nanoflowers for Autophagy Inhibition and Enhanced Antitumor Chemotherapy^† [J]. Chem. J. Chinese Universities, 2020, 41(7): 1461.
[14]	ZHANG Luhao, CAO Shuting, LIU Jiangbo, ZUO Xiaolei, WANG Lihua, FAN Chunhai, LI Jiang. Construction of Controllable Lipid-DNA Complex for Study in Membrane Biology ^† [J]. Chem. J. Chinese Universities, 2020, 41(6): 1151.
[15]	HUANG Yunshuai, YANG Ni, WU Zehong, WU Si. Ruthenium Complexes with Photoresponsive Coordination Bonds for Light-controlled Surface Functions ^† [J]. Chem. J. Chinese Universities, 2020, 41(6): 1174.

Effects of the Dimerization of [Ru(Phen)₂dppz]²⁺ on the Calf Thymus Binding Properties

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments