Purification and Properties of a Novel Se-containing Protein from Se-enriched Ganoderma lucidum and Its Antioxidant Activity

Chem. J. Chinese Universities

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Purification and Properties of a Novel Se-containing Protein from Se-enriched Ganoderma lucidum and Its Antioxidant Activity

DU Ming^1,2, ZHAO Lei¹, LI Chao-Rui¹, ZHAO Guang-Hua¹, HU Xiao-Song¹

1. College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China;
2. College of Food Science and Engineering, Harbin Institute of Technology, Harbin 150001, China

Received:2006-02-10 Revised:1900-01-01 Online:2007-01-10 Published:2007-01-10

Abstract

Abstract:

A novel water-soluble fungi Se-containing protein, named as Se-GL-P, was purified for the first time from Se-enriched GL to establish the relationship between the antioxidant activity of protein and its Se content. This protein was isolated and purified to homogeneity by using ammonium sulfate precipitation followed by two consecutive anion exchange chromatography and size exclusion chromatography. This protein in its native state was identified as a monomer of 36600 estimated by 19.8% weight carbohydrate. The protein is acidic and the N-terminal shows a sequence of DINGGGATLPQKLYLTPDVL, suggesting that this protein belongs to a family of DING protein. The Se content of this protein is 4.87 mg/g protein, and the protein has stronger scavenging activities on superoxide and hydroxyl radicals. It is found that the increasing antioxidant property of this protein depends quantitatively on the increase of its Se content.

Key words: Se-enriched Ganoderma lucidum, Selenium, Se-containing protein, Antioxidant activity

CLC Number:

Q503
O62

TrendMD:

DU Ming^1,2, ZHAO Lei¹, LI Chao-Rui¹, ZHAO Guang-Hua¹, HU Xiao-Song¹. Purification and Properties of a Novel Se-containing Protein from Se-enriched Ganoderma lucidum and Its Antioxidant Activity[J]. Chem. J. Chinese Universities, doi: .

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Purification and Properties of a Novel Se-containing Protein from Se-enriched Ganoderma lucidum and Its Antioxidant Activity

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