Chem. J. Chinese Universities

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Rapid and Sensitive Detection and Characterization of Saccharomyces
cerevisiae by Light Scattering Method

CHEN Shao-Fen; HUANG Cheng-Zhi*; TAN Ke-Jun   

  1. College of Chemistry and Chemical Engineering, Southwest Normal University,
    Chongqing 400715, China
  • Received:2005-11-20 Revised:1900-01-01 Online:2006-06-10 Published:2006-06-10
  • Contact: HUANG Cheng-Zhi

Abstract: The light scattering(LS) spectra of Saccharomyces cerevisiae was studied. The results show that the LS intensities were in proportion to the concentrations of Saccharomyces cerevisiae at peak 308.0 nm in the concentration range of 2.0×104-2.0×106 Cell/mL. The detection limit(3σ) was 4.94x102 Cell/mL. The present new method was successfully applied to the rapid, sensitive determination and characterization of Saccharomyces cerevisiae biomass in suspensions.

Key words: Light scattering assay, Scattering cross section, Absorption cross section, Saccharomyces cerevisiae, Biomass

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