Separation, Purification and Structure of Burdock Oligosaccharide

Abstract

Abstract: A kind of soluble oligosaccharide, Burdock oligosaccharide(BOS₂), was extracted by hot water, deproteinized by enzymolysis and Sevag method, precipated by ethanol, separated and purified by Sephadex G-50 column chromatography from burdock sub-standard root. Its purification, physical and chemical properties and structure were studied by means of some chemical reactions assay and instrumental analysis. The results show that BOS₂ is composed of D-fructose and D-glucose in a molar ratio of about 12∶1, which is confirmed by the composition of 12 fructose residues linked by β(2→1) glucoside bond and 1 glucose residue linked by α(1→2) glucoside bond at the end of linear straight sugar chain. It is shown to be a single homogeneous component.

Key words: Burdock oligosaccharide, Separation and Purification, Structure analysis

CLC Number:

TrendMD:

HAO Lin-Hua, CHEN Lei, ZHONG Na, CHEN Kao-Shan, LI Guang-You. Separation, Purification and Structure of Burdock Oligosaccharide[J]. Chem. J. Chinese Universities, 2005, 26(7): 1242.

References

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