Preparation and Voltammetric Characterization of DNA Modified Electrode with Ethylenediamine as Arm Molecule

Abstract

Abstract: Carboxyl was formed on the surface of glassy carbon electrode(GCE) by electrochemical oxidation. Ethylenediamine(En) was used as the arm molecule to link carboxyl with dsDNA using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride(EDC) and N-hydroxysuccinimide(NHS) as the activators to prepare dsDNA modified electrode(dsDNA/En/GCE). It was shown that dsDNA could be covalently immobilized on the surface of GCE. ssDNA modified electrode(ssDNA/En/GCE) was obtained via the thermal denaturation of dsDNA/En/GCE. The dsDNA/En/GCE and ssDNA/En/GCE were characterized by voltammetry with methylene blue(MB) as the indicator. The results indicated that the currents of the redox peaks of MB at ssDNA/En/GCE were larger than those at dsDNA/En/GCE, and the currents of the redox peaks at En/GCE were the smallest. The peak-currents of MB at the DNA modified electrode had good reproducibility after multi-denaturation and hybridization cycles.

Key words: DNA, Ethylenediamine, Covalent immobilization, Voltammetry, Methylene blue

CLC Number:

O657

TrendMD:

JIAO Kui, XU Gui-Yun, ZHANG Xu-Zhi, MI Chun-Hua . Preparation and Voltammetric Characterization of DNA Modified Electrode with Ethylenediamine as Arm Molecule[J]. Chem. J. Chinese Universities, 2005, 26(5): 841.

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Preparation and Voltammetric Characterization of DNA Modified Electrode with Ethylenediamine as Arm Molecule

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