Studies on Icariin by Electrospray Ionization Mass Spectrometry

Abstract

Abstract: Electrospray ionization mass spectrometry(ESI-MS) in both the positive and the negative ion modes has been used to analyze Icariin(Anhydroicaritin-3-O-α-L-rhamnoside-7-O-β-D-glucopyranoside). The results show that strong peak of [M+2H₂O-H]^- in the negative ion mode was readily observed. Through an experimental method of hydrogen and deuterium exchange, it is proved that the two H₂O is crystal water of Icariin. The collision-induced dissociation fragmentation pathways are different in the positive and the negative ion mode and they are proposed.

Key words: Icariin, Electrospray ionization, Mass spectrometry, H/D exchange

CLC Number:

O657

TrendMD:

DOU Jian-Peng, LIU Zhi-Qiang, LIU Shu-Ying . Studies on Icariin by Electrospray Ionization Mass Spectrometry[J]. Chem. J. Chinese Universities, 2004, 25(5): 837.

References

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