Abstract: The high performance liquid chromatography(HPLC) was applied to investigate the intake and distributional mechanism of 2-allyl phenol in wheat seedling. With a treatment level of 2-allyl phenol at 500 μg/mL, the intake reached 389.67 μg per gram of plant tissue in 72 h(standard deviation, SD= 9.5 ). In comparison with carbofuran(a typical intake reagent) as well as thiram(a typical non-intake reagent), it is for the first time found that 2-allyl phenol could be intaken into wheat seedling. It was transported in a high speed from the root to the top of the seedling. The accumulation levels of 2-allyl phenol in stem(Y₁) and leaves(Y₂) were positively correlated with treatment time(X) in linear relationships of Y₁=2.9586X+44.217 and Y₂=1.6281X-4.4412, respectively. The corresponding coefficients of determination(r²) were 0.848 6 and 0.859 1, respectively. 2-Allyl phenol was distributed in whole seedling at 72 h after the treatment. The accumulation levels at root, stem, and leaves were 99.70(SD=10.58), 243.8(SD=19.87), and 137.37(SD=12.44) μg per gram of plant tissue, respectively. CCCP, temperature, the properties of amino acid and sucrose, as well as the pH of solvents significantly affected the intake of 2-allyl phenol by wheat seedling, indicating that wheat tends to intake and transport 2-allyl phenol actively.

Key words: 2-Allyl phenol, HPLC, Intake transportation, Active intake