Spectroscopic and Electrochemical Studies on the Interaction Between DNAand the Product of the Enzyme-catalyzed Reaction of OAP-H2O2-HRP

Abstract

Abstract: The pure product of 2-aminophenoxazine-3-one was prepared by enzyme-catalyzed reaction and analyzed. The electrochemical behaviour of 2-aminophenoxazine-3-one on a glass carbon electrode was investigated. The interaction between DNA and 2-aminophenoxazine-3-one was investigated by cyclic voltammetry and UV-Vis spectroscopy. The peak potential of 2-aminophenoxazine-3-one in pH 6.8 phosphate buffer solution in the presence of DNA shifts positively, indicating that the bind of 2-aminophenoxazine-3-one with DNA is intercalation action. The influence of reacting time was also studied. The binding ratio of the complex of DNA and 2-aminophenoxazine-3-one at room temperature is calculated to be 1∶1, and the binding constant is to be 6.60×10⁴L/mol.

Key words: 2-Aminophenoxazine-3-one, DNA, Cyclic voltammetry, UV-Vis spectroscopy, Intercalation

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ZHANG Shu-Sheng, NIU Shu-Yan, MA Li-Bo, JIAO Kui . Spectroscopic and Electrochemical Studies on the Interaction Between DNAand the Product of the Enzyme-catalyzed Reaction of OAP-H₂O₂-HRP[J]. Chem. J. Chinese Universities, 2004, 25(4): 630.

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Spectroscopic and Electrochemical Studies on the Interaction Between DNAand the Product of the Enzyme-catalyzed Reaction of OAP-H₂O₂-HRP

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