Configuration and Property Evaluation of a Novel Plastic Capillary Electrophoresis Microchip with Electrochemical Detection System

Abstract

Abstract: A poly(methyl methacrylate)-based capillary electrophoresis microchip, integrated with a microelectrode-based end-column amperometric detection, was described. A short unclosed portion of the separation channel from the detection cell was used as a working-electrode guide channel. With the aid of a three-dimension micropositioner and an optical microscope, a home-made 6 μm carbon-fiber microdisk electrode(tip diameter of 20 μm) was positioned along the guide channel at 30 μm distance from the channel outlet. With dopamine and catechol the as model analytes, the analytical performance of the system was demonstrated and the detection limit of 1.6×10^-7mol/L for dopamine was achieved, to our knowledge, which is the lowest value than that from published literatures using electrophoresis microchip with end-column amperometric detection.

Key words: Poly(methyl methacrylate), Capillary electrophoresis microchip, Electrochemical detection, Microdisk electrode

CLC Number:

O657

TrendMD:

CHEN Zhi-Feng, GAO Yun-Hua, LI Cheng-Wu, LIN Jin-Ming . Configuration and Property Evaluation of a Novel Plastic Capillary Electrophoresis Microchip with Electrochemical Detection System[J]. Chem. J. Chinese Universities, 2004, 25(12): 2253.

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Configuration and Property Evaluation of a Novel Plastic Capillary Electrophoresis Microchip with Electrochemical Detection System

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